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6tjp
From Proteopedia
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==Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve - P212121== | ==Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve - P212121== | ||
| - | <StructureSection load='6tjp' size='340' side='right'caption='[[6tjp]]' scene=''> | + | <StructureSection load='6tjp' size='340' side='right'caption='[[6tjp]], [[Resolution|resolution]] 3.74Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJP OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6tjp]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TJP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6qwp|6qwp]], [[6qx5|6qx5]], [[6qxm|6qxm]], [[6r21|6r21]]</div></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tjp OCA], [https://pdbe.org/6tjp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tjp RCSB], [https://www.ebi.ac.uk/pdbsum/6tjp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tjp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/PORTL_BPT7 PORTL_BPT7]] Forms the portal vertex of the capsid. This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel.[HAMAP-Rule:MF_04120]<ref>PMID:15784250</ref> <ref>PMID:23884409</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Medium-resolution cryo-electron microscopy maps, in particular when they include a significant number of alpha-helices, may allow the building of partial models that are useful for molecular-replacement searches in large crystallographic structures when the structures of homologs are not available and experimental phasing has failed. Here, as an example, the solution of the structure of a bacteriophage portal using a partial 30% model built into a 7.8 A resolution cryo-EM map is shown. Inspection of the self-rotation function allowed the correct oligomerization state to be determined, and density-modification procedures using rotation matrices and a mask based on the cryo-EM structure were critical for solving the structure. A workflow is described that may be applicable to similar cases and this strategy is compared with direct use of the cryo-EM map for molecular replacement. | ||
| + | |||
| + | Using a partial atomic model from medium-resolution cryo-EM to solve a large crystal structure.,Fabrega-Ferrer M, Cuervo A, Fernandez FJ, Machon C, Perez-Luque R, Pous J, Vega MC, Carrascosa JL, Coll M Acta Crystallogr D Struct Biol. 2021 Jan 1;77(Pt 1):11-18. doi:, 10.1107/S2059798320015156. Epub 2021 Jan 1. PMID:33404521<ref>PMID:33404521</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6tjp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bpt7]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Carrascosa | + | [[Category: Carrascosa, J L]] |
| - | [[Category: Coll M]] | + | [[Category: Coll, M]] |
| - | [[Category: Cuervo A]] | + | [[Category: Cuervo, A]] |
| - | [[Category: Fabrega-Ferrer M]] | + | [[Category: Fabrega-Ferrer, M]] |
| - | [[Category: Fernandez | + | [[Category: Fernandez, F J]] |
| - | [[Category: Machon C]] | + | [[Category: Machon, C]] |
| - | [[Category: Perez-Luque R]] | + | [[Category: Perez-Luque, R]] |
| - | [[Category: Pous J]] | + | [[Category: Pous, J]] |
| - | [[Category: Vega | + | [[Category: Vega, M C]] |
| + | [[Category: Dna packaging]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 03:43, 2 July 2021
Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve - P212121
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