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2r09

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{{Seed}}
 
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[[Image:2r09.png|left|200px]]
 
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==Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor==
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The line below this paragraph, containing "STRUCTURE_2r09", creates the "Structure Box" on the page.
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<StructureSection load='2r09' size='340' side='right'caption='[[2r09]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2r09]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R09 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4IP:INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE'>4IP</scene>, <scene name='pdbligand=PE5:3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL'>PE5</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2r09| PDB=2r09 | SCENE= }}
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2r0d|2r0d]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pscd3, Grp1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r09 OCA], [https://pdbe.org/2r09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r09 RCSB], [https://www.ebi.ac.uk/pdbsum/2r09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r09 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/CYH3_MOUSE CYH3_MOUSE]] Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP.<ref>PMID:18042453</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/2r09_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r09 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Arf GTPases regulate membrane trafficking and actin dynamics. Grp1, ARNO, and Cytohesin-1 comprise a family of phosphoinositide-dependent Arf GTPase exchange factors with a Sec7-pleckstrin homology (PH) domain tandem. Here, we report that the exchange activity of the Sec7 domain is potently autoinhibited by conserved elements proximal to the PH domain. The crystal structure of the Grp1 Sec7-PH tandem reveals a pseudosubstrate mechanism of autoinhibition in which the linker region between domains and a C-terminal amphipathic helix physically block the docking sites for the switch regions of Arf GTPases. Mutations within either element result in partial or complete activation. Critical determinants of autoinhibition also contribute to insulin-stimulated plasma membrane recruitment. Autoinhibition can be largely reversed by binding of active Arf6 to Grp1 and by phosphorylation of tandem PKC sites in Cytohesin-1. These observations suggest that Grp1 family GEFs are autoregulated by mechanisms that depend on plasma membrane recruitment for activation.
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===Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor===
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Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors.,DiNitto JP, Delprato A, Gabe Lee MT, Cronin TC, Huang S, Guilherme A, Czech MP, Lambright DG Mol Cell. 2007 Nov 30;28(4):569-83. PMID:18042453<ref>PMID:18042453</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18042453}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2r09" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18042453 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18042453}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2R09 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R09 OCA].
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[[Category: Lk3 transgenic mice]]
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[[Category: Cronin, T C]]
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==Reference==
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[[Category: Czech, M P]]
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<ref group="xtra">PMID:18042453</ref><references group="xtra"/>
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[[Category: Delprato, A]]
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[[Category: Mus musculus]]
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[[Category: DiNitto, J P]]
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[[Category: Cronin, T C.]]
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[[Category: Guilherme, A]]
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[[Category: Czech, M P.]]
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[[Category: Huang, S]]
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[[Category: Delprato, A.]]
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[[Category: Lambright, D G]]
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[[Category: Dinitto, J P.]]
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[[Category: Lee, M T.Gabe]]
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[[Category: Guilherme, A.]]
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[[Category: Huang, S.]]
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[[Category: Lambright, D G.]]
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[[Category: Lee, M T.Gabe.]]
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[[Category: 3-phosphoinositide]]
[[Category: 3-phosphoinositide]]
[[Category: Arf]]
[[Category: Arf]]
[[Category: Autoinhibition]]
[[Category: Autoinhibition]]
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[[Category: Coiled coil]]
 
[[Category: Grp1]]
[[Category: Grp1]]
[[Category: Guanine-nucleotide releasing factor]]
[[Category: Guanine-nucleotide releasing factor]]
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[[Category: Pleckstrin homology domain]]
[[Category: Pleckstrin homology domain]]
[[Category: Signaling protein]]
[[Category: Signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 20:12:06 2009''
 

Current revision

Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor

PDB ID 2r09

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