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2r2i

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(New page: 200px<br /><applet load="2r2i" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r2i, resolution 2.000&Aring;" /> '''Myristoylated Guany...)
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[[Image:2r2i.jpg|left|200px]]<br /><applet load="2r2i" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2r2i, resolution 2.000&Aring;" />
 
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'''Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound'''<br />
 
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==Overview==
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==Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound==
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Guanylate cyclase-activating proteins (GCAPs) are Ca(2+)-binding proteins, myristoylated at the N terminus that regulate guanylate cyclases in, photoreceptor cells and belong to the family of neuronal calcium sensors, (NCS). Many NCS proteins display a recoverin-like "calcium-myristoyl, switch" whereby the myristoyl group, buried inside the protein in the, Ca(2+)-free state, becomes fully exposed upon Ca(2+) binding. Here we, present a 2.0 A resolution crystal structure of myristoylated GCAP1 with, Ca(2+) bound. The acyl group is buried inside Ca(2+)-bound GCAP1. This is, in sharp contrast to Ca(2+)-bound recoverin, where the myristoyl group is, solvent exposed. Furthermore, we provide direct evidence that the acyl, group in GCAP1 remains buried in the Ca(2+)-free state and does not, undergo switching. A pronounced kink in the C-terminal helix and the, presence of the myristoyl group allow clustering of sequence elements, crucial for GCAP1 activity.
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<StructureSection load='2r2i' size='340' side='right'caption='[[2r2i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2r2i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R2I FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GUCA1A, GCAP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2i OCA], [https://pdbe.org/2r2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r2i RCSB], [https://www.ebi.ac.uk/pdbsum/2r2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r2i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/GUC1A_CHICK GUC1A_CHICK]] Stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/2r2i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r2i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Guanylate cyclase-activating proteins (GCAPs) are Ca(2+)-binding proteins myristoylated at the N terminus that regulate guanylate cyclases in photoreceptor cells and belong to the family of neuronal calcium sensors (NCS). Many NCS proteins display a recoverin-like "calcium-myristoyl switch" whereby the myristoyl group, buried inside the protein in the Ca(2+)-free state, becomes fully exposed upon Ca(2+) binding. Here we present a 2.0 A resolution crystal structure of myristoylated GCAP1 with Ca(2+) bound. The acyl group is buried inside Ca(2+)-bound GCAP1. This is in sharp contrast to Ca(2+)-bound recoverin, where the myristoyl group is solvent exposed. Furthermore, we provide direct evidence that the acyl group in GCAP1 remains buried in the Ca(2+)-free state and does not undergo switching. A pronounced kink in the C-terminal helix and the presence of the myristoyl group allow clustering of sequence elements crucial for GCAP1 activity.
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==About this Structure==
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Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1.,Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC Structure. 2007 Nov;15(11):1392-402. PMID:17997965<ref>PMID:17997965</ref>
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2R2I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MYR:'>MYR</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2I OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Stabilizing function for myristoyl group revealed by the crystal structure of a neuronal calcium sensor, guanylate cyclase-activating protein 1., Stephen R, Bereta G, Golczak M, Palczewski K, Sousa MC, Structure. 2007 Nov;15(11):1392-402. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17997965 17997965]
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</div>
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[[Category: Gallus gallus]]
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<div class="pdbe-citations 2r2i" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Stephen, R.]]
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<references/>
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[[Category: BME]]
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__TOC__
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[[Category: CA]]
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</StructureSection>
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[[Category: MYR]]
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[[Category: Chick]]
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[[Category: calcium]]
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[[Category: Large Structures]]
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[[Category: ef hand]]
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[[Category: Stephen, R]]
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[[Category: gcap]]
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[[Category: Calcium]]
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[[Category: gcap-1 ]]
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[[Category: Ef hand]]
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[[Category: gcap1]]
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[[Category: Gcap]]
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[[Category: guanylate cyclase activating protein]]
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[[Category: Gcap-1]]
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[[Category: lipoprotein]]
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[[Category: Gcap1]]
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[[Category: lyase activator]]
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[[Category: Guanylate cyclase activating protein]]
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[[Category: myristate]]
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[[Category: Lipoprotein]]
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[[Category: sensory transduction]]
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[[Category: Lyase activator]]
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[[Category: vision]]
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[[Category: Myristate]]
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[[Category: Sensory transduction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:13:21 2008''
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[[Category: Vision]]

Current revision

Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound

PDB ID 2r2i

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