This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2uz4
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='2uz4' size='340' side='right'caption='[[2uz4]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='2uz4' size='340' side='right'caption='[[2uz4]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2uz4]] is a 3 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2uz4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_10014 Atcc 10014]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UZ4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr> | ||
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[10mh|10mh]], [[1fjx|1fjx]], [[1hmy|1hmy]], [[1m0e|1m0e]], [[1mht|1mht]], [[1skm|1skm]], [[1svu|1svu]], [[2c7o|2c7o]], [[2c7p|2c7p]], [[2c7q|2c7q]], [[2c7r|2c7r]], [[2hmy|2hmy]], [[2uyc|2uyc]], [[2uyh|2uyh]], [[3mht|3mht]], [[4mht|4mht]], [[5mht|5mht]], [[6mht|6mht]], [[7mht|7mht]], [[8mht|8mht]], [[9mht|9mht]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[10mh|10mh]], [[1fjx|1fjx]], [[1hmy|1hmy]], [[1m0e|1m0e]], [[1mht|1mht]], [[1skm|1skm]], [[1svu|1svu]], [[2c7o|2c7o]], [[2c7p|2c7p]], [[2c7q|2c7q]], [[2c7r|2c7r]], [[2hmy|2hmy]], [[2uyc|2uyc]], [[2uyh|2uyh]], [[3mht|3mht]], [[4mht|4mht]], [[5mht|5mht]], [[6mht|6mht]], [[7mht|7mht]], [[8mht|8mht]], [[9mht|9mht]]</div></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uz4 OCA], [https://pdbe.org/2uz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uz4 RCSB], [https://www.ebi.ac.uk/pdbsum/2uz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uz4 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH]] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease. |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Revision as of 10:39, 7 July 2021
HhaI DNA methyltransferase R165N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH
| |||||||||||
