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2vxb

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{{Seed}}
 
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[[Image:2vxb.jpg|left|200px]]
 
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==Structure of the Crb2-BRCT2 domain==
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The line below this paragraph, containing "STRUCTURE_2vxb", creates the "Structure Box" on the page.
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<StructureSection load='2vxb' size='340' side='right'caption='[[2vxb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vxb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VXB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene></td></tr>
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-->
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vxc|2vxc]]</div></td></tr>
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{{STRUCTURE_2vxb| PDB=2vxb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxb OCA], [https://pdbe.org/2vxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vxb RCSB], [https://www.ebi.ac.uk/pdbsum/2vxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/RHP9_SCHPO RHP9_SCHPO]] Essential for cell cycle arrest at the G1 and G2 stages following DNA damage by X-, and UV-irradiation, or inactivation of DNA ligase. Plays a role in the response to DNA damage.<ref>PMID:9153313</ref> <ref>PMID:9407031</ref> <ref>PMID:15550243</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vx/2vxb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vxb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Schizosaccharomyces pombe Crb2 is a checkpoint mediator required for the cellular response to DNA damage. Like human 53BP1 and Saccharomyces cerevisiae Rad9 it contains Tudor(2) and BRCT(2) domains. Crb2-Tudor(2) domain interacts with methylated H4K20 and is required for recruitment to DNA dsDNA breaks. The BRCT(2) domain is required for dimerization, but its precise role in DNA damage repair and checkpoint signaling is unclear. The crystal structure of the Crb2-BRCT(2) domain, alone and in complex with a phosphorylated H2A.1 peptide, reveals the structural basis for dimerization and direct interaction with gamma-H2A.1 in ionizing radiation-induced foci (IRIF). Mutational analysis in vitro confirms the functional role of key residues and allows the generation of mutants in which dimerization and phosphopeptide binding are separately disrupted. Phenotypic analysis of these in vivo reveals distinct roles in the DNA damage response. Dimerization mutants are genotoxin sensitive and defective in checkpoint signaling, Chk1 phosphorylation, and Crb2 IRIF formation, while phosphopeptide-binding mutants are only slightly sensitive to IR, have extended checkpoint delays, phosphorylate Chk1, and form Crb2 IRIF. However, disrupting phosphopeptide binding slows formation of ssDNA-binding protein (Rpa1/Rad11) foci and reduces levels of Rad22(Rad52) recombination foci, indicating a DNA repair defect.
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===STRUCTURE OF THE CRB2-BRCT2 DOMAIN===
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Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair.,Kilkenny ML, Dore AS, Roe SM, Nestoras K, Ho JC, Watts FZ, Pearl LH Genes Dev. 2008 Aug 1;22(15):2034-47. PMID:18676809<ref>PMID:18676809</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18676809}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2vxb" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18676809 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18676809}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Cbs 356]]
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2VXB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXB OCA].
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[[Category: Large Structures]]
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[[Category: Kilkenny, M L]]
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==Reference==
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[[Category: Pearl, L H]]
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Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair., Kilkenny ML, Dore AS, Roe SM, Nestoras K, Ho JC, Watts FZ, Pearl LH, Genes Dev. 2008 Aug 1;22(15):2034-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18676809 18676809]
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[[Category: Roe, S M]]
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[[Category: Schizosaccharomyces pombe]]
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[[Category: Single protein]]
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[[Category: Kilkenny, M L.]]
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[[Category: Pearl, L H.]]
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[[Category: Roe, S M.]]
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[[Category: Brct]]
[[Category: Brct]]
[[Category: Cell cycle]]
[[Category: Cell cycle]]
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[[Category: Nucleus]]
[[Category: Nucleus]]
[[Category: Phosphoprotein]]
[[Category: Phosphoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 13 14:08:36 2008''
 

Current revision

Structure of the Crb2-BRCT2 domain

PDB ID 2vxb

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