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7cpz
From Proteopedia
(Difference between revisions)
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==Crystal structure of Streptoavidin-C1 from Streptomyces cinamonensis== | ==Crystal structure of Streptoavidin-C1 from Streptomyces cinamonensis== | ||
| - | <StructureSection load='7cpz' size='340' side='right'caption='[[7cpz]]' scene=''> | + | <StructureSection load='7cpz' size='340' side='right'caption='[[7cpz]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CPZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7cpz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._h036 Streptomyces sp. h036]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CPZ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cpz OCA], [https://pdbe.org/7cpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cpz RCSB], [https://www.ebi.ac.uk/pdbsum/7cpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cpz ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ADK98_07690 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1519487 Streptomyces sp. H036])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cpz OCA], [https://pdbe.org/7cpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cpz RCSB], [https://www.ebi.ac.uk/pdbsum/7cpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cpz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The members of the avidin protein family are well known for their high affinity towards d-biotin and their structural stability. These properties make avidins a valuable tool for various biotechnological applications. In the present study, two avidin-like biotin-binding proteins (named streptavidin C1 and C2) from Streptomyces cinnamonensis were newly identified while exploring antifungal proteins against Fusarium oxysporum f. sp. cucumerinum. Streptavidin C1 reveals a low correlation (a sequence identity of approximately 64%) with all known streptavidins, whereas streptavidin C2 shares a sequence identity of approximately 94% with other streptavidins. Here, the crystal structures of streptavidin C1 in the mature form and in complex with biotin at 2.1 and 2.5 A resolution, respectively, were assessed. The overall structures present similar tetrameric features with D 2 symmetry to other (strept)avidin structures. Interestingly, the long C-terminal region comprises a short alpha-helix (C-Lid; residues 169-179) and an extension C-terminal peptide (ECP; residues 180-191) which stretches into the biotin-binding sites of the same monomer. This ECP sequence (-(180)VTSANPPAS(188)-) is a newly defined biotin-binding site, which reduces the ability to bind to (strept)avidin family proteins. The novel streptavidin C1 could help in the development of an engineered tetrameric streptavidin with reduced biotin-binding capacity as well as other biomaterial tools. | ||
| + | |||
| + | Insights into the structure of mature streptavidin C1 from Streptomyces cinnamonensis reveal the self-binding of the extension C-terminal peptide to biotin-binding sites.,Jeon BJ, Kim S, Kim MS, Lee JH, Kim BS, Hwang KY IUCrJ. 2021 Jan 11;8(Pt 2):168-177. doi: 10.1107/S2052252520015675. eCollection, 2021 Mar 1. PMID:33708394<ref>PMID:33708394</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7cpz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Hwang | + | [[Category: Streptomyces sp. h036]] |
| - | [[Category: Jeon | + | [[Category: Hwang, K Y]] |
| - | [[Category: Kim | + | [[Category: Jeon, B J]] |
| - | [[Category: Kim S]] | + | [[Category: Kim, M S]] |
| - | [[Category: Lee J | + | [[Category: Kim, S]] |
| + | [[Category: Lee, J H]] | ||
| + | [[Category: Antifungal]] | ||
| + | [[Category: Antimicrobial protein]] | ||
| + | [[Category: Avidin]] | ||
| + | [[Category: Biotin]] | ||
Revision as of 09:12, 21 July 2021
Crystal structure of Streptoavidin-C1 from Streptomyces cinamonensis
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