1exd

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[[Image:1exd.gif|left|200px]]
[[Image:1exd.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1exd |SIZE=350|CAPTION= <scene name='initialview01'>1exd</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1exd", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamine--tRNA_ligase Glutamine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.18 6.1.1.18] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1exd| PDB=1exd | SCENE= }}
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|RELATEDENTRY=[[1qtq|1QTQ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1exd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1exd OCA], [http://www.ebi.ac.uk/pdbsum/1exd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1exd RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE'''
'''CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE'''
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[[Category: Perona, J J.]]
[[Category: Perona, J J.]]
[[Category: Sherlin, L D.]]
[[Category: Sherlin, L D.]]
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[[Category: engineered trna]]
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[[Category: Engineered trna]]
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[[Category: trna aptamer]]
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[[Category: Trna aptamer]]
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[[Category: trna-protein complex]]
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[[Category: Trna-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:37:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:11:26 2008''
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Revision as of 12:37, 2 May 2008

Image:1exd.gif

Template:STRUCTURE 1exd

CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE


Overview

Guided by an in vitro selection experiment designed to obtain tight binding aptamers of Escherichia coli glutamine specific tRNA (tRNAGln) for glutaminyl-tRNA synthetase (GlnRS), we have engineered a tRNA mutant in which the five-nucleotide variable loop sequence 5'-44CAUUC48-3' is replaced by 5'-44AGGU48-3'. This mutant tRNA binds to GlnRS with 30-fold improved affinity compared to the wild type. The 2.7 A cocrystal structure of the RNA aptamer-GlnRS complex reveals major rearrangements in the central tertiary core of the tRNA, while maintaining an RNA-protein interface identical to the wild type. The repacked RNA core features a novel hydrogen bonding arrangement of the trans Levitt pair G15-U48, a new sulfate binding pocket in the major groove, and increased hydrophobic stacking interactions among the bases. These data suggest that enhanced protein binding to a mutant globular RNA can arise from stabilization of RNA tertiary interactions rather than optimization of RNA-protein contacts.

About this Structure

1EXD is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Tertiary core rearrangements in a tight binding transfer RNA aptamer., Bullock TL, Sherlin LD, Perona JJ, Nat Struct Biol. 2000 Jun;7(6):497-504. PMID:10881199 Page seeded by OCA on Fri May 2 15:37:59 2008

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