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1jj8

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Revision as of 06:41, 11 August 2021

Testing the Water-Mediated HIN Recombinase DNA Recognition by Systematic Mutations

Structural highlights

1jj8 is a 3 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	1hcr, 1ijw, 1jj6, 1jko, 1jkp, 1jkq, 1jkr
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HIN_SALTY] A DNA fragment of approximately 900 base pairs, adjacent to the fljB (H2) gene, which specifies the synthesis of phase-2 flagellin, can exist in either orientation with respect to fljB. The orientation of the inversion region controls expression of fljB. The hin gene occupies about two-thirds of the inversion region; it is required for the inversion of the fljB controlling region.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Hin recombinase specifically recognizes its DNA-binding site by means of both major and minor groove interactions. A previous X-ray structure, together with new structures of the Hin DNA-binding domain bound to a recombination half-site that were solved as part of the present study, have revealed that two ordered water molecules are present within the major groove interface. In this report, we test the importance of these waters directly by X-ray crystal structure analysis of complexes with four mutant DNA sequences. These structures, combined with their Hin-binding properties, provide strong support for the critical importance of one of the intermediate waters. A lesser but demonstrable role is ascribed to the second water molecule. The mutant structures also illustrate the prominent roles of thymine methyls both in stabilizing intermediate waters and in interfering with water or amino acid side chain interactions with DNA.

Testing water-mediated DNA recognition by the Hin recombinase.,Chiu TK, Sohn C, Dickerson RE, Johnson RC EMBO J. 2002 Feb 15;21(4):801-14. PMID:11847127^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chiu TK, Sohn C, Dickerson RE, Johnson RC. Testing water-mediated DNA recognition by the Hin recombinase. EMBO J. 2002 Feb 15;21(4):801-14. PMID:11847127 doi:http://dx.doi.org/10.1093/emboj/21.4.801

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jj8"

@@ Line 3: / Line 3: @@
 <StructureSection load='1jj8' size='340' side='right'caption='[[1jj8]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1jj8]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JJ8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1jj8]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJ8 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hcr|1hcr]], [[1ijw|1ijw]], [[1jj6|1jj6]], [[1jko|1jko]], [[1jkp|1jkp]], [[1jkq|1jkq]], [[1jkr|1jkr]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hcr|1hcr]], [[1ijw|1ijw]], [[1jj6|1jj6]], [[1jko|1jko]], [[1jkp|1jkp]], [[1jkq|1jkq]], [[1jkr|1jkr]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jj8 OCA], [http://pdbe.org/1jj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jj8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jj8 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jj8 OCA], [https://pdbe.org/1jj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jj8 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/HIN_SALTY HIN_SALTY]] A DNA fragment of approximately 900 base pairs, adjacent to the fljB (H2) gene, which specifies the synthesis of phase-2 flagellin, can exist in either orientation with respect to fljB. The orientation of the inversion region controls expression of fljB. The hin gene occupies about two-thirds of the inversion region; it is required for the inversion of the fljB controlling region.
+[[https://www.uniprot.org/uniprot/HIN_SALTY HIN_SALTY]] A DNA fragment of approximately 900 base pairs, adjacent to the fljB (H2) gene, which specifies the synthesis of phase-2 flagellin, can exist in either orientation with respect to fljB. The orientation of the inversion region controls expression of fljB. The hin gene occupies about two-thirds of the inversion region; it is required for the inversion of the fljB controlling region.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 31: @@
 ==See Also==
-*[[Resolvase|Resolvase]]
+*[[Resolvase 3D structures|Resolvase 3D structures]]
 == References ==
 <references/>

1jj8

From Proteopedia

Revision as of 06:41, 11 August 2021

Testing the Water-Mediated HIN Recombinase DNA Recognition by Systematic Mutations

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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