This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7etm
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7etm is ON HOLD Authors: Li, Z., Yu, X. Description: C6 portal vertex in the enveloped virion capsid Category: Unreleased Structures [[Category...) |
|||
| (2 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==C6 portal vertex in the enveloped virion capsid== | |
| + | <StructureSection load='7etm' size='340' side='right'caption='[[7etm]], [[Resolution|resolution]] 5.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7etm]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_cytomegalovirus Human cytomegalovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ETM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ETM FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7etm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7etm OCA], [https://pdbe.org/7etm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7etm RCSB], [https://www.ebi.ac.uk/pdbsum/7etm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7etm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/Q6RXD3_HCMV Q6RXD3_HCMV]] Forms a portal in the viral capsid through which viral DNA is translocated during DNA packaging. Assembles as a dodecamer at a single fivefold axe of the T=16 icosahedric capsid. Binds to the molecular motor that translocates the viral DNA, termed terminase.[HAMAP-Rule:MF_04012] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV. The 5-fold symmetric 10-helix anchor-uncommon among known portals-contacts the portal-encircling DNA, which is presumed to squeeze the portal as the genome packaging proceeds. We surmise that the 10-helix anchor dampens this action to delay the portal reaching a "head-full" packaging state, thus facilitating the large genome to be packaged. The 6-fold symmetric turret, latched via a coiled coil to a helix from a major capsid protein, supports the portal to retain the packaged genome. CVSCs at the penton vertices-presumed to increase inner capsid pressure-display a low stoichiometry, which would aid genome retention. We also demonstrate that the portal and capsid undergo conformational changes to facilitate genome ejection after viral cell entry. | ||
| - | + | Structural basis for genome packaging, retention, and ejection in human cytomegalovirus.,Li Z, Pang J, Dong L, Yu X Nat Commun. 2021 Jul 27;12(1):4538. doi: 10.1038/s41467-021-24820-3. PMID:34315863<ref>PMID:34315863</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7etm" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Human cytomegalovirus]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Li, Z]] | [[Category: Li, Z]] | ||
| + | [[Category: Yu, X]] | ||
| + | [[Category: C6 portal vertex]] | ||
| + | [[Category: Enveloped capsid]] | ||
| + | [[Category: Viral protein]] | ||
Current revision
C6 portal vertex in the enveloped virion capsid
| |||||||||||
