1uuk

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(New page: '''Theoretical Model''' The entry 1UUK is a Theoretical Model titled 'PLASMID P1 REPA IS HOMOLOGOUS TO THE F PLASMID REPE CLASS OF INITIATORS.'. Category:Theoretical Model ''Page s...)
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'''Theoretical Model'''
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{{Theoretical_model}}
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The entry 1UUK is a Theoretical Model titled 'PLASMID P1 REPA IS HOMOLOGOUS TO THE F PLASMID REPE CLASS OF INITIATORS.'.
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==PLASMID P1 REPA IS HOMOLOGOUS TO THE F PLASMID REPE CLASS OF INITIATORS.==
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<StructureSection load='1uuk' size='340' side='right'caption='[[1uuk]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UUK FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uuk FirstGlance], [https://www.ebi.ac.uk/pdbsum/1uuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uuk ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA replication of plasmid P1 requires a plasmid-encoded origin DNA-binding protein, RepA. RepA is an inactive dimer and is converted by molecular chaperones into an active monomer that binds RepA binding sites. Although the sequence of RepA is not homologous to that of F plasmid RepE, we found by using fold-recognition programs that RepA shares structural homology with RepE and built a model based on the RepE crystal structure. We constructed mutants in the two predicted DNA binding domains to test the model. As expected, the mutants were defective in P1 DNA binding. The model predicted that RepA binds the first half of the binding site through interactions with the C-terminal DNA binding domain and the second half through interactions with the N-terminal domain. The experiments supported the prediction. The model was further supported by the observation that mutants defective in dimerization map to the predicted subunit interface region, based on the crystal structure of pPS10 RepA, a RepE family member. These results suggest P1 RepA is structurally homologous to plasmid initiators, including those of F, R6K, pSC101, pCU1, pPS10, pFA3, pGSH500, Rts1, RepHI1B, RepFIB, and RSF1010.
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[[Category:Theoretical Model]]
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Plasmid P1 RepA is homologous to the F plasmid RepE class of initiators.,Sharma S, Sathyanarayana BK, Bird JG, Hoskins JR, Lee B, Wickner S J Biol Chem. 2004 Feb 13;279(7):6027-34. Epub 2003 Nov 21. PMID:14634015<ref>PMID:14634015</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 13:29:12 2008''
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</div>
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<div class="pdbe-citations 1uuk" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Theoretical Model]]
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[[Category: Large Structures]]
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[[Category: Bird, J G]]
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[[Category: Hoskins, J R]]
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[[Category: Lee, B]]
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[[Category: Sathyanarayana, B K]]
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[[Category: Sharma, S]]
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[[Category: Wickner, S]]

Current revision

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

PLASMID P1 REPA IS HOMOLOGOUS TO THE F PLASMID REPE CLASS OF INITIATORS.

PDB ID 1uuk

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