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2mcm

From Proteopedia

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{{Seed}}
 
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[[Image:2mcm.png|left|200px]]
 
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==MACROMOMYCIN==
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The line below this paragraph, containing "STRUCTURE_2mcm", creates the "Structure Box" on the page.
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<StructureSection load='2mcm' size='340' side='right'caption='[[2mcm]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2mcm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_macromomyceticus"_hamada_and_okami "streptomyces macromomyceticus" hamada and okami]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MCM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MCM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mcm OCA], [https://pdbe.org/2mcm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mcm RCSB], [https://www.ebi.ac.uk/pdbsum/2mcm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mcm ProSAT]</span></td></tr>
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{{STRUCTURE_2mcm| PDB=2mcm | SCENE= }}
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/MACM_STRMA MACM_STRMA]] Binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/2mcm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2mcm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of macromomycin, the apoprotein of the antitumor antibiotic auromomycin, has been determined and refined at 1.6-A resolution. The overall structure is composed of a flattened seven-stranded antiparallel beta-barrel and two antiparallel beta-sheet ribbons. The barrel and the ribbons define a deep cleft that is the chromophore binding site. The cleft is very accessible and in this structure is occupied by two 2-methyl-2,4-pentanediol and two water molecules. The overall shape of the binding site is similar to that of the analogue actinoxanthin. Highly specific side chains that are not conserved between different analogues extend into the binding site and may be important to the chromophore binding specificity.
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===MACROMOMYCIN===
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Crystal structure analysis of auromomycin apoprotein (macromomycin) shows importance of protein side chains to chromophore binding selectivity.,Van Roey P, Beerman TA Proc Natl Acad Sci U S A. 1989 Sep;86(17):6587-91. PMID:2771945<ref>PMID:2771945</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_2771945}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2mcm" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 2771945 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_2771945}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Streptomyces macromomyceticus hamada and okami]]
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2MCM is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_macromomyceticus Streptomyces macromomyceticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MCM OCA].
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[[Category: Large Structures]]
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[[Category: Roey, P Van]]
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==Reference==
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<ref group="xtra">PMID:2771945</ref><references group="xtra"/>
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[[Category: Streptomyces macromomyceticus]]
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[[Category: Vanroey, P.]]
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[[Category: Apoprotein]]
[[Category: Apoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 19:09:58 2009''
 

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MACROMOMYCIN

PDB ID 2mcm

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