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2mcm

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(New page: 200px<br /><applet load="2mcm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2mcm, resolution 1.5&Aring;" /> '''MACROMOMYCIN'''<br />...)
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[[Image:2mcm.jpg|left|200px]]<br /><applet load="2mcm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2mcm, resolution 1.5&Aring;" />
 
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'''MACROMOMYCIN'''<br />
 
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==Overview==
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==MACROMOMYCIN==
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The crystal structure of macromomycin, the apoprotein of the antitumor, antibiotic auromomycin, has been determined and refined at 1.6-A, resolution. The overall structure is composed of a flattened, seven-stranded antiparallel beta-barrel and two antiparallel beta-sheet, ribbons. The barrel and the ribbons define a deep cleft that is the, chromophore binding site. The cleft is very accessible and in this, structure is occupied by two 2-methyl-2,4-pentanediol and two water, molecules. The overall shape of the binding site is similar to that of the, analogue actinoxanthin. Highly specific side chains that are not conserved, between different analogues extend into the binding site and may be, important to the chromophore binding specificity.
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<StructureSection load='2mcm' size='340' side='right'caption='[[2mcm]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2mcm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_macromomyceticus"_hamada_and_okami "streptomyces macromomyceticus" hamada and okami]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MCM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MCM FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mcm OCA], [https://pdbe.org/2mcm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mcm RCSB], [https://www.ebi.ac.uk/pdbsum/2mcm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mcm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/MACM_STRMA MACM_STRMA]] Binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mc/2mcm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2mcm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of macromomycin, the apoprotein of the antitumor antibiotic auromomycin, has been determined and refined at 1.6-A resolution. The overall structure is composed of a flattened seven-stranded antiparallel beta-barrel and two antiparallel beta-sheet ribbons. The barrel and the ribbons define a deep cleft that is the chromophore binding site. The cleft is very accessible and in this structure is occupied by two 2-methyl-2,4-pentanediol and two water molecules. The overall shape of the binding site is similar to that of the analogue actinoxanthin. Highly specific side chains that are not conserved between different analogues extend into the binding site and may be important to the chromophore binding specificity.
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==About this Structure==
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Crystal structure analysis of auromomycin apoprotein (macromomycin) shows importance of protein side chains to chromophore binding selectivity.,Van Roey P, Beerman TA Proc Natl Acad Sci U S A. 1989 Sep;86(17):6587-91. PMID:2771945<ref>PMID:2771945</ref>
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2MCM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_macromomyceticus Streptomyces macromomyceticus] with CA, MRD and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2MCM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure analysis of auromomycin apoprotein (macromomycin) shows importance of protein side chains to chromophore binding selectivity., Van Roey P, Beerman TA, Proc Natl Acad Sci U S A. 1989 Sep;86(17):6587-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2771945 2771945]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2mcm" style="background-color:#fffaf0;"></div>
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[[Category: Streptomyces macromomyceticus]]
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== References ==
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[[Category: Vanroey, P.]]
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<references/>
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[[Category: CA]]
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__TOC__
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[[Category: MPD]]
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</StructureSection>
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[[Category: MRD]]
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[[Category: Streptomyces macromomyceticus hamada and okami]]
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[[Category: apoprotein]]
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[[Category: Large Structures]]
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[[Category: Roey, P Van]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:43:37 2007''
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[[Category: Apoprotein]]

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MACROMOMYCIN

PDB ID 2mcm

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