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1xak

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Revision as of 16:35, 27 October 2021

STRUCTURE OF THE SARS-CORONAVIRUS ORF7A ACCESSORY PROTEIN

Structural highlights

1xak is a 1 chain structure with sequence from Cvhsa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[NS7A_CVHSA] Non-structural protein which is dispensable for virus replication in cell culture.^[1]

Publication Abstract from PubMed

The open reading frame (ORF) 7a of the SARS-associated coronavirus (SARS-CoV) encodes a unique type I transmembrane protein of unknown function. We have determined the 1.8 A resolution crystal structure of the N-terminal ectodomain of orf7a, revealing a compact seven-stranded beta sandwich unexpectedly similar in fold and topology to members of the Ig superfamily. We also demonstrate that, in SARS-CoV- infected cells, the orf7a protein is expressed and retained intracellularly. Confocal microscopy studies using orf7a and orf7a/CD4 chimeras implicate the short cytoplasmic tail and transmembrane domain in trafficking of the protein within the endoplasmic reticulum and Golgi network. Taken together, our findings provide a structural and cellular framework in which to explore the role of orf7a in SARS-CoV pathogenesis.

Structure and intracellular targeting of the SARS-coronavirus Orf7a accessory protein.,Nelson CA, Pekosz A, Lee CA, Diamond MS, Fremont DH Structure. 2005 Jan;13(1):75-85. PMID:15642263^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tan YJ, Fielding BC, Goh PY, Shen S, Tan TH, Lim SG, Hong W. Overexpression of 7a, a protein specifically encoded by the severe acute respiratory syndrome coronavirus, induces apoptosis via a caspase-dependent pathway. J Virol. 2004 Dec;78(24):14043-7. PMID:15564512 doi:http://dx.doi.org/10.1128/JVI.78.24.14043-14047.2004
↑ Nelson CA, Pekosz A, Lee CA, Diamond MS, Fremont DH. Structure and intracellular targeting of the SARS-coronavirus Orf7a accessory protein. Structure. 2005 Jan;13(1):75-85. PMID:15642263 doi:http://dx.doi.org/S0969-2126(04)00384-3

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xak"

@@ Line 3: / Line 3: @@
 <StructureSection load='1xak' size='340' side='right'caption='[[1xak]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1xak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XAK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1xak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAK FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xak OCA], [http://pdbe.org/1xak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xak RCSB], [http://www.ebi.ac.uk/pdbsum/1xak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xak ProSAT], [http://www.topsan.org/Proteins/MCSG/1xak TOPSAN]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xak OCA], [https://pdbe.org/1xak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xak RCSB], [https://www.ebi.ac.uk/pdbsum/1xak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xak ProSAT], [https://www.topsan.org/Proteins/MCSG/1xak TOPSAN]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NS7A_CVHSA NS7A_CVHSA]] Non-structural protein which is dispensable for virus replication in cell culture.<ref>PMID:15564512</ref>
+[[https://www.uniprot.org/uniprot/NS7A_CVHSA NS7A_CVHSA]] Non-structural protein which is dispensable for virus replication in cell culture.<ref>PMID:15564512</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

1xak

From Proteopedia

Revision as of 16:35, 27 October 2021

STRUCTURE OF THE SARS-CORONAVIRUS ORF7A ACCESSORY PROTEIN

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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