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1y2n

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{{Theoretical_model}}
{{Theoretical_model}}
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[[Image:1y2n.png|left|200px]]
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==MODEL STRUCTURE OF TRASTUZUMAB COMPLEXED WITH H98, A MIMIC ANTIGEN EPITOPE OF HER-2==
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<StructureSection load='1y2n' size='340' side='right'caption='[[1y2n]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2N FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2n FirstGlance], [https://www.ebi.ac.uk/pdbsum/1y2n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2n ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Trastuzumab, a humanized antibody to HER-2, has been shown to be effective in the treatment of breast cancer in which HER-2 overexpression and metastasis occurs. In our search for an effective mimic epitope of HER-2 binding with trastuzumab and to develop HER-2 peptide vaccine, we screened a phage display 12-mer peptide library with trastuzumab as the target. A mimetic peptide (mimotope) H98 (LLGPYELWELSH) that could specifically recognize trastuzumab was isolated. The DNA encoding peptide H98 was cloned and expressed as the fusion protein GST-H98 in Escherichia coli BL21. The purified GST-H98 could specifically bind to trastuzumab and block the binding of trastuzumab to HER-2 protein. Moreover, H98 could significantly block the function of trastuzumab inhibiting the growth of cancer cells. Mice that were immunized with GST-H98 made specific antibody to H98 as well as to HER-2. In addition, T-cell proliferation occurred in mice immunized with GST-H98. Although no sequence homology was found between H98 and HER-2, through the use of structure analysis we were able to determine that peptide H98 contributed to a conformational epitope of HER-2. Furthermore, we determined that the last two amino acids at the C terminus, and the third together with the fourth amino acid at the N terminus of peptide H98 are critical to the binding of H98 to trastuzumab. As a result, we conclude that peptide H98 has potential for being developed as a HER-2 vaccine for biotherapy of cancer with HER-2 overexpression.
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{{STRUCTURE_1y2n| PDB=1y2n | SCENE= }}
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A novel peptide isolated from a phage display peptide library with trastuzumab can mimic antigen epitope of HER-2.,Jiang B, Liu W, Qu H, Meng L, Song S, Ouyang T, Shou C J Biol Chem. 2005 Feb 11;280(6):4656-62. Epub 2004 Nov 9. PMID:15536075<ref>PMID:15536075</ref>
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===MODEL STRUCTURE OF TRASTUZUMAB COMPLEXED WITH H98, A MIMIC ANTIGEN EPITOPE OF HER-2===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_15536075}}
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<div class="pdbe-citations 1y2n" style="background-color:#fffaf0;"></div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015536075</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Theoretical Model]]
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[[Category: Large Structures]]
[[Category: Jiang, B]]
[[Category: Jiang, B]]
[[Category: Liu, W]]
[[Category: Liu, W]]

Current revision

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

MODEL STRUCTURE OF TRASTUZUMAB COMPLEXED WITH H98, A MIMIC ANTIGEN EPITOPE OF HER-2

PDB ID 1y2n

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