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2wur

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Revision as of 16:45, 3 November 2021

Atomic resolution structure of GFP measured on a rotating anode

Structural highlights

2wur is a 1 chain structure with sequence from Aeqvi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	2emn, 1jc0, 2fzu, 1rm9, 1ema, 1rmo, 1f09, 1yhg, 1kp5, 1q73, 2b3q, 1z1p, 1yhh, 1rmm, 2b3p, 1emm, 2fwq, 1emg, 1rmp, 1emb, 2emd, 1q4e, 1q4d, 1kys, 1c4f, 1q4a, 1h6r, 1eml, 1w7t, 1emk, 2emo, 2wsn, 1q4b, 1w7u, 1yjf, 1q4c, 1yfp, 1emf, 1yj2, 2ah8, 2wso, 1yhi, 1eme, 1cv7, 1gfl, 1qyq, 1jbz, 1kyr, 2aha, 1rrx, 1qxt, 1myw, 2yfp, 1qy3, 1b9c, 1w7s, 1jby, 1f0b, 1jc1, 1qyo, 1bfp, 1huy, 1hcj, 1emc, 1kyp, 1z1q, 1qyf
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

"Proton-collecting antenna" are conjectured to consist of several carboxylates within hydrogen-bond (HB) networks on the surface of proteins, which funnel protons to the orifice of an internal proton wire leading to the protein's active site. Yet such constructions were never directly visualized. Here we report an X-ray structure of green fluorescent protein (GFP) of the highest resolution to date (0.9 A). It allows the identification of some pivotal hydrogen atoms pertinent to uncertainties concerning the protonation state of the chromophore. Applying a computer algorithm for mapping proton wires in proteins reveals the previously observed "active site wire" connecting Glu222 with the surface carboxylate Glu5. In addition, it is now possible to identify what appears to be a proton-collecting apparatus of GFP. It consists of a negative surface patch containing carboxylates, threonines, and water molecules, connected by a HB network to Glu5. Furthermore, we detect exit points via Asn146 and His148 to a hydrophobic surface region. The more extensive HB network of the present structure, as compared with earlier GFP structures, is not accidental. A systematic investigation of over 100 mutants shows a clear correlation between the observed water content of GFP X-ray structures and their resolution. With increasing water content, the proton wires become progressively larger. These findings corroborate the scenario in which the photodissociated proton from wild-type GFP can leak outside, whereafter another proton is recruited via the proton-collecting apparatus reported herein.

Visualizing proton antenna in a high-resolution green fluorescent protein structure.,Shinobu A, Palm GJ, Schierbeek AJ, Agmon N J Am Chem Soc. 2010 Aug 18;132(32):11093-102. PMID:20698675^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shinobu A, Palm GJ, Schierbeek AJ, Agmon N. Visualizing proton antenna in a high-resolution green fluorescent protein structure. J Am Chem Soc. 2010 Aug 18;132(32):11093-102. PMID:20698675 doi:10.1021/ja1010652

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wur"

@@ Line 3: / Line 3: @@
 <StructureSection load='2wur' size='340' side='right'caption='[[2wur]], [[Resolution|resolution]] 0.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wur]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WUR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wur]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WUR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WUR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2emn|2emn]], [[1jc0|1jc0]], [[2fzu|2fzu]], [[1rm9|1rm9]], [[1ema|1ema]], [[1rmo|1rmo]], [[1f09|1f09]], [[1yhg|1yhg]], [[1kp5|1kp5]], [[1q73|1q73]], [[2b3q|2b3q]], [[1z1p|1z1p]], [[1yhh|1yhh]], [[1rmm|1rmm]], [[2b3p|2b3p]], [[1emm|1emm]], [[2fwq|2fwq]], [[1emg|1emg]], [[1rmp|1rmp]], [[1emb|1emb]], [[2emd|2emd]], [[1q4e|1q4e]], [[1q4d|1q4d]], [[1kys|1kys]], [[1c4f|1c4f]], [[1q4a|1q4a]], [[1h6r|1h6r]], [[1eml|1eml]], [[1w7t|1w7t]], [[1emk|1emk]], [[2emo|2emo]], [[2wsn|2wsn]], [[1q4b|1q4b]], [[1w7u|1w7u]], [[1yjf|1yjf]], [[1q4c|1q4c]], [[1yfp|1yfp]], [[1emf|1emf]], [[1yj2|1yj2]], [[2ah8|2ah8]], [[2wso|2wso]], [[1yhi|1yhi]], [[1eme|1eme]], [[1cv7|1cv7]], [[1gfl|1gfl]], [[1qyq|1qyq]], [[1jbz|1jbz]], [[1kyr|1kyr]], [[2aha|2aha]], [[1rrx|1rrx]], [[1qxt|1qxt]], [[1myw|1myw]], [[2yfp|2yfp]], [[1qy3|1qy3]], [[1b9c|1b9c]], [[1w7s|1w7s]], [[1jby|1jby]], [[1f0b|1f0b]], [[1jc1|1jc1]], [[1qyo|1qyo]], [[1bfp|1bfp]], [[1huy|1huy]], [[1hcj|1hcj]], [[1emc|1emc]], [[1kyp|1kyp]], [[1z1q|1z1q]], [[1qyf|1qyf]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2emn|2emn]], [[1jc0|1jc0]], [[2fzu|2fzu]], [[1rm9|1rm9]], [[1ema|1ema]], [[1rmo|1rmo]], [[1f09|1f09]], [[1yhg|1yhg]], [[1kp5|1kp5]], [[1q73|1q73]], [[2b3q|2b3q]], [[1z1p|1z1p]], [[1yhh|1yhh]], [[1rmm|1rmm]], [[2b3p|2b3p]], [[1emm|1emm]], [[2fwq|2fwq]], [[1emg|1emg]], [[1rmp|1rmp]], [[1emb|1emb]], [[2emd|2emd]], [[1q4e|1q4e]], [[1q4d|1q4d]], [[1kys|1kys]], [[1c4f|1c4f]], [[1q4a|1q4a]], [[1h6r|1h6r]], [[1eml|1eml]], [[1w7t|1w7t]], [[1emk|1emk]], [[2emo|2emo]], [[2wsn|2wsn]], [[1q4b|1q4b]], [[1w7u|1w7u]], [[1yjf|1yjf]], [[1q4c|1q4c]], [[1yfp|1yfp]], [[1emf|1emf]], [[1yj2|1yj2]], [[2ah8|2ah8]], [[2wso|2wso]], [[1yhi|1yhi]], [[1eme|1eme]], [[1cv7|1cv7]], [[1gfl|1gfl]], [[1qyq|1qyq]], [[1jbz|1jbz]], [[1kyr|1kyr]], [[2aha|2aha]], [[1rrx|1rrx]], [[1qxt|1qxt]], [[1myw|1myw]], [[2yfp|2yfp]], [[1qy3|1qy3]], [[1b9c|1b9c]], [[1w7s|1w7s]], [[1jby|1jby]], [[1f0b|1f0b]], [[1jc1|1jc1]], [[1qyo|1qyo]], [[1bfp|1bfp]], [[1huy|1huy]], [[1hcj|1hcj]], [[1emc|1emc]], [[1kyp|1kyp]], [[1z1q|1z1q]], [[1qyf|1qyf]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wur OCA], [http://pdbe.org/2wur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wur RCSB], [http://www.ebi.ac.uk/pdbsum/2wur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wur ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wur OCA], [https://pdbe.org/2wur PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wur RCSB], [https://www.ebi.ac.uk/pdbsum/2wur PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wur ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
+[[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2wur

From Proteopedia

Revision as of 16:45, 3 November 2021

Atomic resolution structure of GFP measured on a rotating anode

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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