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2x19

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{{Seed}}
 
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[[Image:2x19.jpg|left|200px]]
 
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==Crystal structure of Importin13 - RanGTP complex==
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The line below this paragraph, containing "STRUCTURE_2x19", creates the "Structure Box" on the page.
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<StructureSection load='2x19' size='340' side='right'caption='[[2x19]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x19]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X19 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2x19| PDB=2x19 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x19 OCA], [https://pdbe.org/2x19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x19 RCSB], [https://www.ebi.ac.uk/pdbsum/2x19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x19 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/GSP1_YEAST GSP1_YEAST]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Essential for cell viability. By analogy with Ras, Ran may be activated when GTP is exchanged for bound GDP by RCC1 and inactivated when GTP is hydrolyzed by Ran upon activation by RanGAP1. [[https://www.uniprot.org/uniprot/IPO13_HUMAN IPO13_HUMAN]] Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13.<ref>PMID:11447110</ref> <ref>PMID:15143176</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x1/2x19_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x19 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mago and Y14 are core components of the exon junction complex (EJC), an assembly central to nonsense-mediated mRNA decay in humans and mRNA localization in flies. The Mago-Y14 heterodimer shuttles between the nucleus, where it is loaded onto specific mRNAs, and the cytoplasm, where it functions in translational regulation. The heterodimer is imported back into the nucleus by Importin 13 (Imp13), a member of the karyopherin-beta family of transport factors. We have elucidated the structural basis of the Mago-Y14 nuclear import cycle. The 3.35 A structure of the Drosophila Imp13-Mago-Y14 complex shows that Imp13 forms a ring-like molecule, reminiscent of Crm1, and encircles the Mago-Y14 cargo with a conserved interaction surface. The 2.8 A structure of human Imp13 bound to RanGTP reveals how Mago-Y14 is released in the nucleus by a steric hindrance mechanism. Comparison of the two structures suggests how this unusual karyopherin might function in bidirectional nucleocytoplasmic transport.
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===CRYSTAL STRUCTURE OF IMPORTIN13 - RANGTP COMPLEX===
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Nuclear import mechanism of the EJC component Mago-Y14 revealed by structural studies of importin 13.,Bono F, Cook AG, Grunwald M, Ebert J, Conti E Mol Cell. 2010 Jan 29;37(2):211-22. PMID:20122403<ref>PMID:20122403</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2x19" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20122403}}, adds the Publication Abstract to the page
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*[[Importin 3D structures|Importin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20122403 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20122403}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2X19 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X19 OCA].
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[[Category: Bono, F]]
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[[Category: Conti, E]]
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==Reference==
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[[Category: Cook, A G]]
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<ref group="xtra">PMID:20122403</ref><references group="xtra"/>
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[[Category: Ebert, J]]
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[[Category: Homo sapiens]]
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[[Category: Gruenwald, M]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Bono, F.]]
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[[Category: Conti, E.]]
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[[Category: Cook, A G.]]
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[[Category: Ebert, J.]]
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[[Category: Gruenwald, M.]]
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[[Category: Nuclear transport]]
[[Category: Nuclear transport]]
[[Category: Protein transport]]
[[Category: Protein transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 10:22:14 2010''
 

Current revision

Crystal structure of Importin13 - RanGTP complex

PDB ID 2x19

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