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2ejy

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[[Image:2ejy.jpg|left|200px]]
 
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{{Structure
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==Solution structure of the p55 PDZ T85C domain complexed with the glycophorin C F127C peptide==
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|PDB= 2ejy |SIZE=350|CAPTION= <scene name='initialview01'>2ejy</scene>
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<StructureSection load='2ejy' size='340' side='right'caption='[[2ejy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2ejy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EJY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ev8|2ev8]]</div></td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ejy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejy OCA], [https://pdbe.org/2ejy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ejy RCSB], [https://www.ebi.ac.uk/pdbsum/2ejy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ejy ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[2ev8|2EV8]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejy OCA], [http://www.ebi.ac.uk/pdbsum/2ejy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ejy RCSB]</span>
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[[https://www.uniprot.org/uniprot/EM55_HUMAN EM55_HUMAN]] Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity). [[https://www.uniprot.org/uniprot/GLPC_HUMAN GLPC_HUMAN]] This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Solution structure of the p55 PDZ T85C domain complexed with the glycophorin C F127C peptide'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/2ejy_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ejy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
p55, a member of the membrane-associated guanylate kinase family, includes a PDZ domain that specifically interacts with the C-terminal region of glycophorin C in the ternary complex of p55, protein 4.1 and glycophorin C. Here we present the first NMR-derived complex structure of the p55 PDZ domain and the C-terminal peptide of glycophorin C, obtained by using a threonine to cysteine (T85C) mutant of the p55 PDZ domain and a phenylalanine to cysteine (F127C) mutant of the glycophorin C peptide. Our NMR results revealed that the two designed mutant molecules retain the specific interaction manner that exists between the wild type molecules and can facilitate the structure determination by NMR, due to the stable complex formation via an intermolecular disulfide bond. The complex structure provides insight into the specific interaction of the p55 PDZ domain with the two key residues, Ile128 and Tyr126, of glycophorin C.
p55, a member of the membrane-associated guanylate kinase family, includes a PDZ domain that specifically interacts with the C-terminal region of glycophorin C in the ternary complex of p55, protein 4.1 and glycophorin C. Here we present the first NMR-derived complex structure of the p55 PDZ domain and the C-terminal peptide of glycophorin C, obtained by using a threonine to cysteine (T85C) mutant of the p55 PDZ domain and a phenylalanine to cysteine (F127C) mutant of the glycophorin C peptide. Our NMR results revealed that the two designed mutant molecules retain the specific interaction manner that exists between the wild type molecules and can facilitate the structure determination by NMR, due to the stable complex formation via an intermolecular disulfide bond. The complex structure provides insight into the specific interaction of the p55 PDZ domain with the two key residues, Ile128 and Tyr126, of glycophorin C.
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==About this Structure==
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Structural insight into the interaction between the p55 PDZ domain and glycophorin C.,Kusunoki H, Kohno T Biochem Biophys Res Commun. 2007 Aug 10;359(4):972-8. Epub 2007 Jun 11. PMID:17572384<ref>PMID:17572384</ref>
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2EJY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJY OCA].
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==Reference==
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Structural insight into the interaction between the p55 PDZ domain and glycophorin C., Kusunoki H, Kohno T, Biochem Biophys Res Commun. 2007 Aug 10;359(4):972-8. Epub 2007 Jun 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17572384 17572384]
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[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Kohno, T.]]
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[[Category: Kusunoki, H.]]
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[[Category: gpc]]
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[[Category: maguk]]
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[[Category: membrane protein]]
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[[Category: p55]]
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[[Category: pdz]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:50:14 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ejy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Human]]
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[[Category: Large Structures]]
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[[Category: Kohno, T]]
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[[Category: Kusunoki, H]]
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[[Category: Gpc]]
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[[Category: Maguk]]
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[[Category: Membrane protein]]
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[[Category: P55]]
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[[Category: Pdz]]

Current revision

Solution structure of the p55 PDZ T85C domain complexed with the glycophorin C F127C peptide

PDB ID 2ejy

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