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2rp3

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{{Seed}}
 
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[[Image:2rp3.png|left|200px]]
 
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==Solution Structure of Cyanovirin-N Domain B Mutant==
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The line below this paragraph, containing "STRUCTURE_2rp3", creates the "Structure Box" on the page.
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<StructureSection load='2rp3' size='340' side='right'caption='[[2rp3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2rp3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_variabilis_var._ellipsospora Anabaena variabilis var. ellipsospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RP3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RP3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ezm|2ezm]]</div></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rp3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rp3 OCA], [https://pdbe.org/2rp3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rp3 RCSB], [https://www.ebi.ac.uk/pdbsum/2rp3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rp3 ProSAT]</span></td></tr>
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{{STRUCTURE_2rp3| PDB=2rp3 | SCENE= }}
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL]] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rp/2rp3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rp3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The cyanobacterial lectin Cyanovirin-N (CV-N) exhibits antiviral activity against HIV at a low nanomolar concentration by interacting with high-mannose oligosaccharides on the virus surface envelope glycoprotein gp120. Atomic structures of wild-type CV-N revealed a monomer in solution and a domain-swapped dimer in the crystal, with the monomer comprising two independent carbohydrate binding sites that individually bind with micromolar affinity to di- and trimannoses. In the mutant CVN(mutDB), the binding site on domain B was abolished and the protein was found to be completely inactive against HIV. We determined the solution NMR and crystal structures of this variant and characterized its sugar binding properties. In solution and the crystal, CVN(mutDB) is a monomer and no domain-swapping was observed. The protein binds to Man-3 and Man-9 with similar dissociation constants ( approximately 4 muM). This confirms that the nanomolar activity of wild-type CV-N is related to the multisite nature of the protein carbohydrate interaction.
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===Solution Structure of Cyanovirin-N Domain B Mutant===
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Solution and crystal structures of a sugar binding site mutant of cyanovirin-N: no evidence of domain swapping.,Matei E, Furey W, Gronenborn AM Structure. 2008 Aug 6;16(8):1183-94. PMID:18682220<ref>PMID:18682220</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18682220}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2rp3" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18682220 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_18682220}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Anabaena variabilis var. ellipsospora]]
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2RP3 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RP3 OCA].
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[[Category: Large Structures]]
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[[Category: Furey, W]]
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==Reference==
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[[Category: Gronenborn, A M]]
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<ref group="xtra">PMID:18682220</ref><references group="xtra"/>
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[[Category: Matei, E]]
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[[Category: Nostoc ellipsosporum]]
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[[Category: Antiviral protein]]
[[Category: Antiviral protein]]
[[Category: Cyanovirin-n]]
[[Category: Cyanovirin-n]]
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[[Category: Hiv-inactivating]]
[[Category: Hiv-inactivating]]
[[Category: Monomer]]
[[Category: Monomer]]
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[[Category: Nmr]]
 
[[Category: No 3d domain-swapping]]
[[Category: No 3d domain-swapping]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 02:55:52 2009''
 

Current revision

Solution Structure of Cyanovirin-N Domain B Mutant

PDB ID 2rp3

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