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2z4h
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:2z4h.png|left|200px]] | ||
| - | < | + | ==Crystal structure of the Cpx pathway activator NlpE from Escherichia coli== |
| - | + | <StructureSection load='2z4h' size='340' side='right'caption='[[2z4h]], [[Resolution|resolution]] 2.80Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2z4h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z4H FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |
| - | - | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2z4i|2z4i]]</div></td></tr> |
| - | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nlpE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4h OCA], [https://pdbe.org/2z4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z4h RCSB], [https://www.ebi.ac.uk/pdbsum/2z4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/NLPE_ECOLI NLPE_ECOLI]] Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR.<ref>PMID:15252048</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z4h_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z4h ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | NlpE, an outer membrane lipoprotein, functions during envelope stress responses in Gram-negative bacteria. In Escherichia coli, adhesion to abiotic surfaces has been reported to activate the Cpx pathway in an NlpE-dependent manner. External copper ions are also thought to activate the Cpx pathway mediated by NlpE. We determined the crystal structure of NlpE from E. coli at 2.6 A resolution. The structure showed that NlpE consists of two beta barrel domains. The N-terminal domain resembles the bacterial lipocalin Blc, and the C-terminal domain has an oligonucleotide/oligosaccharide-binding (OB) fold. From both biochemical analyses and the crystal structure, it can be deduced that the cysteine residues in the CXXC motif may be chemically active. Furthermore, two monomers in the asymmetric unit form an unusual 3D domain-swapped dimer. These findings indicate that tertiary and/or quaternary structural instability may be responsible for Cpx pathway activation. | ||
| - | + | Structural studies of the Cpx pathway activator NlpE on the outer membrane of Escherichia coli.,Hirano Y, Hossain MM, Takeda K, Tokuda H, Miki K Structure. 2007 Aug;15(8):963-76. PMID:17698001<ref>PMID:17698001</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2z4h" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| - | + | *[[Copper homeostasis protein|Copper homeostasis protein]] | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | == | + | [[Category: Bacillus coli migula 1895]] |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Hirano, Y]] | |
| - | == | + | [[Category: Hossain, M M]] |
| - | + | [[Category: Miki, K]] | |
| - | [[Category: | + | [[Category: Takeda, K]] |
| - | [[Category: | + | [[Category: Tokuda, H]] |
| - | [[Category: Hirano, Y | + | |
| - | [[Category: Hossain, M M | + | |
| - | [[Category: Miki, K | + | |
| - | [[Category: Takeda, K | + | |
| - | [[Category: Tokuda, H | + | |
[[Category: 3d domain swapping]] | [[Category: 3d domain swapping]] | ||
[[Category: Beta barrel]] | [[Category: Beta barrel]] | ||
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[[Category: Outer memblane lipoprotein]] | [[Category: Outer memblane lipoprotein]] | ||
[[Category: Signaling protein activator]] | [[Category: Signaling protein activator]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:39:58 2008'' | ||
Current revision
Crystal structure of the Cpx pathway activator NlpE from Escherichia coli
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