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3jsv

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{{Seed}}
 
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[[Image:3jsv.jpg|left|200px]]
 
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==Crystal structure of mouse NEMO CoZi in complex with Lys63-linked di-ubiquitin==
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The line below this paragraph, containing "STRUCTURE_3jsv", creates the "Structure Box" on the page.
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<StructureSection load='3jsv' size='340' side='right'caption='[[3jsv]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3jsv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JSV FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3f89|3f89]], [[2zvo|2zvo]], [[2zvn|2zvn]], [[3fx0|3fx0]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ikbkg, Nemo, NEMO(249-343) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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{{STRUCTURE_3jsv| PDB=3jsv | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jsv OCA], [https://pdbe.org/3jsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jsv RCSB], [https://www.ebi.ac.uk/pdbsum/3jsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jsv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/NEMO_MOUSE NEMO_MOUSE]] Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination (By similarity).<ref>PMID:9927690</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/3jsv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jsv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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NEMO is essential for activation of the NF-kappaB signaling pathway, which is regulated by ubiquitination of proteins. The C-terminal leucine zipper of NEMO and its adjacent coiled-coil region (CC2-LZ) reportedly bind to linear ubiquitin chains with 1 microM affinity and to Lys 63-linked chains with 100 microM affinity. Here we report the crystal structure of the CC2-LZ region of mouse NEMO in complex with Lys 63-linked di-ubiquitin (K63-Ub(2)) at 2.7A resolution. The ubiquitin-binding region consists of a 130A-long helix and forms a parallel coiled-coil dimer. The Ile 44-centered hydrophobic patch of ubiquitin is recognized in the middle of the NEMO ubiquitin-binding region. NEMO interacts with each K63-Ub(2)via a single ubiquitin-binding site, consistent with low affinity binding with K63-Ub(2).
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===Crystal structure of mouse NEMO CoZi in complex with Lys63-linked di-ubiquitin===
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Crystal structure of the NEMO ubiquitin-binding domain in complex with Lys 63-linked di-ubiquitin.,Yoshikawa A, Sato Y, Yamashita M, Mimura H, Yamagata A, Fukai S FEBS Lett. 2009 Oct 20;583(20):3317-22. Epub 2009 Sep 18. PMID:19766637<ref>PMID:19766637</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3jsv" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19766637}}, adds the Publication Abstract to the page
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*[[3D structures of ubiquitin|3D structures of ubiquitin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19766637 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19766637}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human]]
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3JSV is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JSV OCA].
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[[Category: Large Structures]]
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[[Category: Lk3 transgenic mice]]
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==Reference==
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[[Category: Fukai, S]]
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<ref group="xtra">PMID:19766637</ref><references group="xtra"/>
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[[Category: Mimura, H]]
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[[Category: Homo sapiens]]
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[[Category: Sato, Y]]
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[[Category: Mus musculus]]
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[[Category: Yamagata, A]]
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[[Category: Fukai, S.]]
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[[Category: Yamashita, M]]
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[[Category: Mimura, H.]]
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[[Category: Yoshikawa, A]]
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[[Category: Sato, Y.]]
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[[Category: Yamagata, A.]]
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[[Category: Yamashita, M.]]
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[[Category: Yoshikawa, A.]]
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[[Category: Cellular signaling]]
[[Category: Cellular signaling]]
[[Category: Coiled coil]]
[[Category: Coiled coil]]
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[[Category: Nucleus]]
[[Category: Nucleus]]
[[Category: Phosphoprotein]]
[[Category: Phosphoprotein]]
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[[Category: Signaling protein/transcription complex]]
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[[Category: Signaling protein-transcription complex]]
[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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[[Category: Zinc]]
[[Category: Zinc]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 28 13:31:43 2009''
 

Current revision

Crystal structure of mouse NEMO CoZi in complex with Lys63-linked di-ubiquitin

PDB ID 3jsv

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