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NAD synthase

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<StructureSection load='' size='350' side='right' caption='NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry [[1xng]])' scene='54/541072/Cv/1'>
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{{STRUCTURE_1ee1| PDB=1ee1 | SIZE=350| SCENE= |right|CAPTION= NAD+ synthase complex with Mg+2 (green), ATP (stick model) and deamino-NAD [[1ee1]] }}
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== Function ==
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'''NAD+ synthase''' or '''NH3-dependent NAD synthetase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH<sub>3</sub> to AMP, NAD+ and diphosphate (PP). NADS is part of the NAD synthesis pathway<ref>PMID:12898714</ref> . See [[NAD]].
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'''NAD+ synthase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH3 to AMP, NAD+ and diphophate (PP). NADS is part of the NAD synthesis pathway.
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== Structural highlights ==
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The <scene name='54/541072/Cv/8'>ATP binding site</scene> contains <scene name='54/541072/Cv/9'>Mg+2 ion</scene>. The <scene name='54/541072/Cv/10'>NAD binding site is located in the interface of the 2 subunits</scene><ref>PMID:15645437</ref>. Water molecules are shown as red spheres.
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</StructureSection>
== 3D Structures of NAD+ synthase ==
== 3D Structures of NAD+ synthase ==
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{{#tree:id=OrganizedByTopic|openlevels=0|
{{#tree:id=OrganizedByTopic|openlevels=0|
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*'''NADS synthase'''
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*'''NAD synthase'''
**[[3p52]] – NADS – ''Campylobacter jejuni'' <br />
**[[3p52]] – NADS – ''Campylobacter jejuni'' <br />
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**[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br />
**[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br />
**[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br />
**[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br />
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**[[6kv3]] – NADS – ''Staphylococcus aureus''<br />
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**[[5wp0]] – NADS – ''Allivibrio fischeri''<br />
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**[[5huh]], [[5huj]] – NADS – ''Streptococcus pyogenes''<br />
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**[[3n05]] – NADS – ''Streptomyces avermitillis''<br />
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**[[4xfd]] – PaNADS – ''Pseudomonas aeruginosa''<br />
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**[[4q16]] – NADS – ''Deinococcus radiodurans''<br />
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**[[2e18]] – NADS – ''Pyrococcus horikoshii''<br />
*NADS binary complex
*NADS binary complex
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**[[1wxh]] – EcNADS + NAD <br />
**[[1wxh]] – EcNADS + NAD <br />
**[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br />
**[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br />
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**[[6c8q]] – NADS + NAD – ''Enterococcus faecalis''<br />
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**[[5f23]] – PaNADS + NAD <br />
*NADS ternary complex
*NADS ternary complex
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**[[1wxg]] – EcNADS + Mg + deamino-NAD <br />
**[[1wxg]] – EcNADS + Mg + deamino-NAD <br />
**[[2pz8]] – BaNADS + Mg + ATP derivative <br />
**[[2pz8]] – BaNADS + Mg + ATP derivative <br />
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**[[3fiu]] – NADS + Mg + AMP – ''Francisella tularensis''<br />
*NADS quaternary complex
*NADS quaternary complex
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**[[1nsy]] – BsNADS + Mg + ATP + ADP + PP <br />
**[[1nsy]] – BsNADS + Mg + ATP + ADP + PP <br />
**[[2nsy]] – BsNADS + Mg + AMP + NAD + PP <br />
**[[2nsy]] – BsNADS + Mg + AMP + NAD + PP <br />
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*NADS glutamine-dependent
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**[[6ofb]] – hNADS + AMP + NAD derivative<br />
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**[[3sdb]] – MtNADS (mutant) – ''Mycobacterium tuberculosis'' <br />
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**[[3seq]], [[3sez]] – MtNADS (mutant) + ATP derivative + pyridinium derivative <br />
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**[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br />
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**[[3syt]] – MtNADS + AMP + NAD + glutamate <br />
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**[[3dla]] – MtNADS + norleucine + deamido-NAD <br />
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**[[6ofc]] – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate <br />
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**[[4f4h]] – NADS – ''Burkholderia thailandensis''<br />
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**[[3ilv]] – NADS – ''Cytophaga hutchinsonii''<br />
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}}
}}
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry 1xng)

Drag the structure with the mouse to rotate

3D Structures of NAD+ synthase

Updated on 15-November-2021

References

  1. ↑ Suda Y, Tachikawa H, Yokota A, Nakanishi H, Yamashita N, Miura Y, Takahashi N. Saccharomyces cerevisiae QNS1 codes for NAD(+) synthetase that is functionally conserved in mammals. Yeast. 2003 Aug;20(11):995-1005. PMID:12898714 doi:http://dx.doi.org/10.1002/yea.1008
  2. ↑ Kang GB, Kim YS, Im YJ, Rho SH, Lee JH, Eom SH. Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori. Proteins. 2005 Mar 1;58(4):985-8. PMID:15645437 doi:10.1002/prot.20377

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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