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7oz6
From Proteopedia
(Difference between revisions)
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==Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MC)== | ==Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MC)== | ||
| - | <StructureSection load='7oz6' size='340' side='right'caption='[[7oz6]]' scene=''> | + | <StructureSection load='7oz6' size='340' side='right'caption='[[7oz6]], [[Resolution|resolution]] 1.76Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OZ6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7oz6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OZ6 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oz6 OCA], [https://pdbe.org/7oz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oz6 RCSB], [https://www.ebi.ac.uk/pdbsum/7oz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oz6 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7os3|7os3]], [[7os5|7os5]], [[7os6|7os6]], [[7ou1|7ou1]]</div></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oz6 OCA], [https://pdbe.org/7oz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oz6 RCSB], [https://www.ebi.ac.uk/pdbsum/7oz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oz6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rhizobium etli, a nitrogen-fixing bacterial symbiont of legume plants, encodes an essential L-asparaginase (ReAV) with no sequence homology to known enzymes with this activity. High-resolution crystal structures of ReAV show indeed a structurally distinct, dimeric enzyme, with some resemblance to glutaminases and beta-lactamases. However, ReAV has no glutaminase or lactamase activity, and at pH 9 its allosteric asparaginase activity is relatively high, with Km for L-Asn at 4.2 mM and kcat of 438 s(-1). The active site of ReAV, deduced from structural comparisons and confirmed by mutagenesis experiments, contains a highly specific Zn(2+) binding site without a catalytic role. The extensive active site includes residues with unusual chemical properties. There are two Ser-Lys tandems, all connected through a network of H-bonds to the Zn center, and three tightly bound water molecules near Ser48, which clearly indicate the catalytic nucleophile. | ||
| + | |||
| + | Crystal structures of the elusive Rhizobium etli L-asparaginase reveal a peculiar active site.,Loch JI, Imiolczyk B, Sliwiak J, Wantuch A, Bejger M, Gilski M, Jaskolski M Nat Commun. 2021 Nov 18;12(1):6717. doi: 10.1038/s41467-021-27105-x. PMID:34795296<ref>PMID:34795296</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7oz6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Gilski M]] | + | [[Category: Gilski, M]] |
| - | [[Category: Imiolczyk B]] | + | [[Category: Imiolczyk, B]] |
| - | [[Category: Jaskolski M]] | + | [[Category: Jaskolski, M]] |
| - | [[Category: Loch | + | [[Category: Loch, J I]] |
| + | [[Category: Amidohydrolase]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: L-asparaginase]] | ||
| + | [[Category: Rhizobium etli]] | ||
Revision as of 06:32, 1 December 2021
Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MC)
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