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2kny

From Proteopedia

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{{Seed}}
 
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[[Image:2kny.jpg|left|200px]]
 
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==Fusion construct of CR17 from LRP-1 and ApoE residues 130-149==
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The line below this paragraph, containing "STRUCTURE_2kny", creates the "Structure Box" on the page.
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<StructureSection load='2kny' size='340' side='right'caption='[[2kny]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kny]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KNY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LRP1, A2MR, APR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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{{STRUCTURE_2kny| PDB=2kny | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kny OCA], [https://pdbe.org/2kny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kny RCSB], [https://www.ebi.ac.uk/pdbsum/2kny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kny ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/LRP1_HUMAN LRP1_HUMAN]] Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.<ref>PMID:1702392</ref> <ref>PMID:1618748</ref> <ref>PMID:11907044</ref> <ref>PMID:12888553</ref> <ref>PMID:12713657</ref> Functions as a receptor for Pseudomonas aeruginosa exotoxin A.<ref>PMID:1702392</ref> <ref>PMID:1618748</ref> <ref>PMID:11907044</ref> <ref>PMID:12888553</ref> <ref>PMID:12713657</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/2kny_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kny ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Clusters of complement-type ligand-binding repeats (CRs) in the low-density lipoprotein receptor (LDLR) family are thought to mediate the interactions with their various ligands. Apolipoprotein E (ApoE), a key ligand for cholesterol homeostasis, has been shown to interact with LDLR-related protein 1 (LRP) through these clusters. The segment comprising the receptor-binding portion of ApoE (residues 130-149) has been found to have a weak affinity for isolated CRs. We have fused this region of ApoE to a high-affinity CR from LRP (CR17) for structural elucidation of the complex. The interface reveals a motif that has previously been observed in CR domains with other binding partners, but with several novel features. Comparison to free CR17 reveals that very few structural changes result from this binding event, but significant changes in intrinsic dynamics are observed upon binding. NMR perturbation experiments suggest that this interface may be similar to several other ligand interactions with LDLRs.
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===Fusion construct of CR17 from LRP-1 and ApoE residues 130-149===
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Structure of the minimal interface between ApoE and LRP.,Guttman M, Prieto JH, Handel TM, Domaille PJ, Komives EA J Mol Biol. 2010 Apr 30;398(2):306-19. Epub 2010 Mar 19. PMID:20303980<ref>PMID:20303980</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20303980}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2kny" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20303980 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20303980}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human]]
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2KNY is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KNY OCA].
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[[Category: Large Structures]]
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[[Category: Guttman, M]]
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==Reference==
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[[Category: Komives, E A]]
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<ref group="xtra">PMID:20303980</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
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[[Category: Guttman, M.]]
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[[Category: Komives, E A.]]
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[[Category: Apoe]]
[[Category: Apoe]]
[[Category: Calcium]]
[[Category: Calcium]]
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[[Category: Receptor]]
[[Category: Receptor]]
[[Category: Transmembrane]]
[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 14 09:35:18 2010''
 

Current revision

Fusion construct of CR17 from LRP-1 and ApoE residues 130-149

PDB ID 2kny

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