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| | ==Crystal structure of Arabidopsis CnfU involved in iron-sulfur cluster biosynthesis== | | ==Crystal structure of Arabidopsis CnfU involved in iron-sulfur cluster biosynthesis== |
| - | <StructureSection load='2z51' size='340' side='right' caption='[[2z51]], [[Resolution|resolution]] 1.35Å' scene=''> | + | <StructureSection load='2z51' size='340' side='right'caption='[[2z51]], [[Resolution|resolution]] 1.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2z51]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z51 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2z51]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z51 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NIFU2, CNFU2, NFU2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NIFU2, CNFU2, NFU2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z51 OCA], [http://pdbe.org/2z51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z51 RCSB], [http://www.ebi.ac.uk/pdbsum/2z51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z51 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z51 OCA], [https://pdbe.org/2z51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z51 RCSB], [https://www.ebi.ac.uk/pdbsum/2z51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z51 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NIFU2_ARATH NIFU2_ARATH]] Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins. Required for biogenesis of ferredoxin, a major photosynthetic electron carrier containing [2Fe-2S] cluster. Required for the assembly of photosystem I complex.<ref>PMID:15031412</ref> <ref>PMID:15361144</ref> | + | [[https://www.uniprot.org/uniprot/NIFU2_ARATH NIFU2_ARATH]] Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins. Required for biogenesis of ferredoxin, a major photosynthetic electron carrier containing [2Fe-2S] cluster. Required for the assembly of photosystem I complex.<ref>PMID:15031412</ref> <ref>PMID:15361144</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Arabidopsis thaliana]] | | [[Category: Arabidopsis thaliana]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Nakai, M]] | | [[Category: Nakai, M]] |
| | [[Category: Yabe, T]] | | [[Category: Yabe, T]] |
| Structural highlights
Function
[NIFU2_ARATH] Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins. Required for biogenesis of ferredoxin, a major photosynthetic electron carrier containing [2Fe-2S] cluster. Required for the assembly of photosystem I complex.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
CnfU, a key iron-sulfur (Fe-S) cluster biosynthetic scaffold that is required for biogenesis of ferredoxin and photosystem I in chloroplasts, consists of two tandemly repeated domains in which only the N-terminal domain contains a conserved CXXC motif. We have determined the crystal structure of the metal-free dimer of AtCnfU-V from Arabidopsis thaliana at 1.35 A resolution. The N-terminal domains of the two monomers are linked together through two intermolecular disulfide bonds between the CXXC motifs. At the dimer interface, a total of four cysteine sulfur atoms provide a Fe-S cluster assembly site surrounded by uncharged but hydrophilic structurally mobile segments. The C-terminal domain of one monomer interacts with the N-terminal domain of the opposing monomer and thereby stabilizes dimer formation. Furthermore, Fe K-edge X-ray absorption spectroscopic analysis of the holo-CnfU dimer in solution suggests the presence of a typical [2Fe-2S]-type cluster coordinated by four thiolate ligands. Based on these data, a plausible model of the holo-AtCnfU-V dimer containing a surface-exposed [2Fe-2S] cluster assembled in the dimer interface was deduced. We propose that such a structural framework is important for CnfU to function as a Fe-S cluster biosynthetic scaffold.
Structural analysis of Arabidopsis CnfU protein: an iron-sulfur cluster biosynthetic scaffold in chloroplasts.,Yabe T, Yamashita E, Kikuchi A, Morimoto K, Nakagawa A, Tsukihara T, Nakai M J Mol Biol. 2008 Aug 1;381(1):160-73. Epub 2008 Jun 4. PMID:18585737[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yabe T, Morimoto K, Kikuchi S, Nishio K, Terashima I, Nakai M. The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem I. Plant Cell. 2004 Apr;16(4):993-1007. Epub 2004 Mar 18. PMID:15031412 doi:http://dx.doi.org/10.1105/tpc.020511
- ↑ Touraine B, Boutin JP, Marion-Poll A, Briat JF, Peltier G, Lobreaux S. Nfu2: a scaffold protein required for [4Fe-4S] and ferredoxin iron-sulphur cluster assembly in Arabidopsis chloroplasts. Plant J. 2004 Oct;40(1):101-11. PMID:15361144 doi:http://dx.doi.org/10.1111/j.1365-313X.2004.02189.x
- ↑ Yabe T, Yamashita E, Kikuchi A, Morimoto K, Nakagawa A, Tsukihara T, Nakai M. Structural analysis of Arabidopsis CnfU protein: an iron-sulfur cluster biosynthetic scaffold in chloroplasts. J Mol Biol. 2008 Aug 1;381(1):160-73. Epub 2008 Jun 4. PMID:18585737 doi:10.1016/j.jmb.2008.05.072
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