Phosphoribosylaminoimidazole carboxylase

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<StructureSection load='1d7a' size='350' side='right' caption='Structure of E. coli PurE octamer complex with 5-aminoimidazole ribonucleotide (AIR) (PDB entry [[1d7a]])' scene='57/571253/Cv/1'>
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<StructureSection load='' size='350' side='right' caption='Structure of E. coli PurE octamer complex with 5-aminoimidazole ribonucleotide (AIR) (PDB entry [[1d7a]])' scene='57/571253/Cv/1'>
== Function ==
== Function ==
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'''Phosphoribosylaminoimidazole carboxylase (PurE)''' is part of the purine biosynthesis and catalyses the reversible conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 5-aminoimidazole ribonucleotide (AIR). In bacteria PurE and PurK or '''Phosphoribosylaminoimidazole carboxylase ATPase subunit''' are independent enzymes while in plants and fungi it is a domain of phosphoribosylaminoimidazole carboxylase<ref>PMID:1534690</ref>. '''PurK''' catalyzes the conversion of AIR,ATP and bicarbonate to N5-CAIR, ADP and phosphate.
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'''Phosphoribosylaminoimidazole carboxylase (PurE)''' or '''N5-carboxyaminoimidazole ribonucleotide mutase''' is part of the purine biosynthesis and catalyzes the reversible conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 5-aminoimidazole ribonucleotide (AIR). In bacteria PurE and '''PurK''' or '''Phosphoribosylaminoimidazole carboxylase ATPase subunit''' are independent enzymes while in plants and fungi it is a domain of phosphoribosylaminoimidazole carboxylase<ref>PMID:1534690</ref>. '''PurK''' catalyzes the conversion of AIR,ATP and bicarbonate to N5-CAIR, ADP and phosphate.
== Structural highlights ==
== Structural highlights ==
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The biological assembly of E. coli PurE is <scene name='57/571253/Cv/2'>octamer</scene>. PurE <scene name='57/571253/Cv/3'>active site</scene> is located in a <scene name='57/571253/Cv/4'>cleft between 3 subunits</scene><ref>PMID:10574791</ref>. Water molecule shown as red sphere.
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The biological assembly of ''E. coli'' PurE is <scene name='57/571253/Cv/5'>octamer</scene>. PurE <scene name='57/571253/Cv/6'>active site</scene> is located in a <scene name='57/571253/Cv/7'>cleft between 3 subunits</scene><ref>PMID:10574791</ref>. Water molecule is shown as red sphere.
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</StructureSection>
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==3D structures of PurE==
==3D structures of PurE==
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[[Phosphoribosylaminoimidazole carboxylase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*PurE
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**[[1qcz]] – EcPurE – ''Escherichia coli''<br />
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**[[1o4v]] – TmPurE – ''Thermotoga maritima''<br />
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**[[1u11]], [[2fw1]] - AaPurE – ''Acetobacter aceti''<br />
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**[[2fw6]], [[2fw7]], [[2fw8]], [[2fw9]], [[2fwa]], [[2fwb]] - AaPurE (mutant)<br />
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**[[1xmp]], [[4ay3]], [[4ay4]], [[4b4k]] - BaPurE – ''Bacillus anthracis''<br />
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**[[2ywx]] – PurE – ''Methanocaldococcus jannaschii''<br />
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**[[3kuu]] - PurE (mutant) – ''Yersinia pestis''<br />
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**[[3lp6]] - PurE (mutant) – ''Mycobacterium tuberculosis''<br />
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**[[3oow]] – FtPurE – ''Francesella tularensis''<br />
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**[[3rg8]] – TdPurE – ''Treponema denticola''<br />
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**[[5c5d]] - TdPurE (mutant) <br />
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**[[3trh]] – PurE – ''Coxiell burnetii''<br />
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**[[4ja0]] – PurE – silkworm<br />
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**[[3ors]] – SaPurE – ''Staphylococcus aureus''<br />
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**[[4grd]] – PurE – ''Burkholderia cenocepacia''<br />
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**[[4e4t]] – PurE ATPase subunit – ''Burkholderia ambifatia''<br />
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*PurE binary complex
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**[[2fwi]], [[2fwj]] - AaPurE (mutant) + AIR<br />
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**[[1d7a]] – EcPurE + AIR<br />
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**[[3rgg]] – TdPurE + AIR<br />
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**[[2fwp]] - AaPurE (mutant) + iso-CAIR<br />
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**[[2ate]] - EcPurE + nitro-AIR<br />
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**[[2nsh]] - EcPurE (mutant) + nitro-AIR<br />
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**[[2nsj]], [[2nsl]] - EcPurE (mutant) + CAIR<br />
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**[[3opq]] – FtPurE + fructose-6-phosphate<br />
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*PurK
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**[[4m9u]] – FtPurK <br />
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**[[4izo]] – PurK – ''Burkholderia thailandensis''<br />
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**[[3q2o]] – BaPurK <br />
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**[[3orr]] – SaPurK <br />
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**[[2z04]] – AaPurK – ''Aquifex aeolicus'' <br />
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**[[1b6r]] – EcPurK <br />
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*PurK complex
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**[[5jqw]], [[4mam]] – FtPurK + ADP analog<br />
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**[[4ma0]] – FtPurK + ATP<br />
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**[[4ma5]] – FtPurK + AMP-PNP<br />
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**[[3v4s]] – BaPurK + ATP + ADP + CO3<br />
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**[[4dlk]] – BaPurK + ATP<br />
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**[[3qff]] – BaPurK + ADP<br />
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**[[3r5h]] – BaPurK + ADP + AIR<br />
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**[[3ax6]] – TmPurK + ADP<br />
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**[[3aw8]] – PurK + AMP – ''Thermos thermophilus''<br />
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**[[3orq]] – SaPurK + ADP<br />
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**[[3eth]] – EcPurK + ATP<br />
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**[[1b6s]] – EcPurK + ADP<br />
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**[[3etj]] – EcPurK + ADP + Pi<br />
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of E. coli PurE octamer complex with 5-aminoimidazole ribonucleotide (AIR) (PDB entry 1d7a)

Drag the structure with the mouse to rotate

References

  1. ↑ Meyer E, Leonard NJ, Bhat B, Stubbe J, Smith JM. Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway. Biochemistry. 1992 Jun 2;31(21):5022-32. PMID:1534690
  2. ↑ Mathews II, Kappock TJ, Stubbe J, Ealick SE. Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway. Structure. 1999 Nov 15;7(11):1395-406. PMID:10574791

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

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