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3c8g
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| - | [[Image:3c8g.jpg|left|200px]]<br /><applet load="3c8g" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="3c8g, resolution 2.50Å" /> | ||
| - | '''Crystal structure of a possible transciptional regulator YggD from Shigella flexneri 2a str. 2457T'''<br /> | ||
| - | == | + | ==Crystal structure of a possible transciptional regulator YggD from Shigella flexneri 2a str. 2457T== |
| - | + | <StructureSection load='3c8g' size='340' side='right'caption='[[3c8g]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | [ | + | == Structural highlights == |
| - | [[ | + | <table><tr><td colspan='2'>[[3c8g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2hkt 2hkt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C8G FirstGlance]. <br> |
| - | [[ | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> |
| - | [[ | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yggD, S3120, SF2919 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr> | |
| - | [ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8g OCA], [https://pdbe.org/3c8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c8g RCSB], [https://www.ebi.ac.uk/pdbsum/3c8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c8g ProSAT], [https://www.topsan.org/Proteins/MCSG/3c8g TOPSAN]</span></td></tr> |
| - | [ | + | </table> |
| - | [ | + | == Evolutionary Conservation == |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| - | [ | + | Check<jmol> |
| - | + | <jmolCheckbox> | |
| - | [ | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/3c8g_consurf.spt"</scriptWhenChecked> |
| - | [[ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | + | <text>to colour the structure by Evolutionary Conservation</text> | |
| - | + | </jmolCheckbox> | |
| - | [ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c8g ConSurf]. |
| - | [[ | + | <div style="clear:both"></div> |
| - | [ | + | <div style="background-color:#fffaf0;"> |
| - | + | == Publication Abstract from PubMed == | |
| + | Many bacteria express phosphoenolpyruvate-dependent phosphotransferase systems (PTS). The mannitol-specific PTS catalyze the uptake and phosphorylation of d-mannitol. The uptake system comprises several genes encoded in the single operon. The expression of the mannitol operon is regulated by a proposed transcriptional factor, mannitol operon repressor (MtlR) that was first studied in Escherichia coli. Here we report the first crystal structures of MtlR from Vibrio parahemeolyticus (Vp-MtlR) and its homolog YggD protein from Shigella flexneri (Sf-YggD). MtlR and YggD belong to the same protein family (Pfam05068). Although Vp-MtlR and Sf-YggD share low sequence identity (22%), their overall structures are very similar, representing a novel all alpha-helical fold, and indicate similar function. However, their lack of any known DNA-binding structural motifs and their unfavorable electrostatic properties imply that MtlR/YggD are unlikely to bind a specific DNA operator directly as proposed earlier. This structural observation is further corroborated by in vitro DNA-binding studies of E. coli MtlR (Ec-MtlR), which detected no interaction of Ec-MtlR with the well characterized mannitol operator/promoter region. Therefore, MtlR/YggD belongs to a new class of transcription factors in bacteria that may regulate gene expression indirectly as a part of a larger transcriptional complex. | ||
| - | + | The mannitol operon repressor MtlR belongs to a new class of transcription regulators in bacteria.,Tan K, Clancy S, Borovilos M, Zhou M, Horer S, Moy S, Volkart LL, Sassoon J, Baumann U, Joachimiak A J Biol Chem. 2009 Dec 25;284(52):36670-9. Epub 2009 Oct 19. PMID:19840941<ref>PMID:19840941</ref> | |
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3c8g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Shigella flexneri 2a str. 2457t]] | ||
| + | [[Category: Borovilos, M]] | ||
| + | [[Category: Clancy, S]] | ||
| + | [[Category: Joachimiak, A]] | ||
| + | [[Category: Structural genomic]] | ||
| + | [[Category: Tan, K]] | ||
| + | [[Category: Apc27974]] | ||
| + | [[Category: Mannitol operon repressor]] | ||
| + | [[Category: Mcsg]] | ||
| + | [[Category: Methylation]] | ||
| + | [[Category: PSI, Protein structure initiative]] | ||
| + | [[Category: Transcription regulator]] | ||
| + | [[Category: Yggd]] | ||
Current revision
Crystal structure of a possible transciptional regulator YggD from Shigella flexneri 2a str. 2457T
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