2kv1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:36, 2 February 2022) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2kv1.jpg|left|200px]]
 
-
<!--
+
==Insights into Function, Catalytic Mechanism and Fold Evolution of Mouse Selenoprotein Methionine Sulfoxide Reductase B1 through Structural Analysis==
-
The line below this paragraph, containing "STRUCTURE_2kv1", creates the "Structure Box" on the page.
+
<StructureSection load='2kv1' size='340' side='right'caption='[[2kv1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2kv1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2kao 2kao]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KV1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kv1 OCA], [https://pdbe.org/2kv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kv1 RCSB], [https://www.ebi.ac.uk/pdbsum/2kv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kv1 ProSAT]</span></td></tr>
-
{{STRUCTURE_2kv1| PDB=2kv1 | SCENE= }}
+
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/2kv1_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kv1 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Methionine sulfoxide reductases protect cells by repairing oxidatively damaged methionine residues in proteins. Here, we report the first three-dimensional structure of the mammalian selenoprotein methionine sulfoxide reductase B1 (MsrB1), determined by high resolution NMR spectroscopy. Heteronuclear multidimensional spectra yielded NMR spectral assignments for the reduced form of MsrB1 in which catalytic selenocysteine (Sec) was replaced with cysteine (Cys). MsrB1 consists of a central structured core of two beta-sheets and a highly flexible, disordered N-terminal region. Analysis of pH dependence of NMR signals of catalytically relevant residues, comparison with the data for bacterial MsrBs, and NMR-based structural analysis of methionine sulfoxide (substrate) and methionine sulfone (inhibitor) binding to MsrB1 at the atomic level reveal a mechanism involving catalytic Sec(95) and resolving Cys(4) residues in catalysis. The MsrB1 structure differs from the structures of Cys-containing MsrBs in the use of distal selenenylsulfide, residues needed for catalysis, and the mode in which the active form of the enzyme is regenerated. In addition, this is the first structure of a eukaryotic zinc-containing MsrB, which highlights the structural role of this metal ion bound to four conserved Cys. We integrated this information into a structural model of evolution of MsrB superfamily.
-
===Insights into Function, Catalytic Mechanism and Fold Evolution of Mouse Selenoprotein Methionine Sulfoxide Reductase B1 through Structural Analysis===
+
Insights into function, catalytic mechanism, and fold evolution of selenoprotein methionine sulfoxide reductase B1 through structural analysis.,Aachmann FL, Sal LS, Kim HY, Marino SM, Gladyshev VN, Dikiy A J Biol Chem. 2010 Oct 22;285(43):33315-23. Epub 2010 Jul 5. PMID:20605785<ref>PMID:20605785</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
==About this Structure==
+
</div>
-
2KV1 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2kao 2kao]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV1 OCA].
+
<div class="pdbe-citations 2kv1" style="background-color:#fffaf0;"></div>
-
[[Category: Mus musculus]]
+
== References ==
-
[[Category: Aachmann, F L.]]
+
<references/>
-
[[Category: Dikiy, A.]]
+
__TOC__
-
[[Category: Gladyshev, V N.]]
+
</StructureSection>
-
[[Category: Kim, H.]]
+
[[Category: Large Structures]]
-
[[Category: Sal, L S.]]
+
[[Category: Lk3 transgenic mice]]
 +
[[Category: Aachmann, F L]]
 +
[[Category: Dikiy, A]]
 +
[[Category: Gladyshev, V N]]
 +
[[Category: Kim, H Y]]
 +
[[Category: Sal, L S]]
[[Category: Metal-binding]]
[[Category: Metal-binding]]
[[Category: Msrb1]]
[[Category: Msrb1]]
Line 27: Line 43:
[[Category: Selenium]]
[[Category: Selenium]]
[[Category: Selr]]
[[Category: Selr]]
-
[[Category: Zinc]]
 
- 
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 17 09:22:00 2010''
 

Current revision

Insights into Function, Catalytic Mechanism and Fold Evolution of Mouse Selenoprotein Methionine Sulfoxide Reductase B1 through Structural Analysis

PDB ID 2kv1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kv1"
Personal tools