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3d0q
From Proteopedia
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| - | [[Image:3d0q.jpg|left|200px]] | ||
| - | < | + | ==Crystal structure of calG3 from Micromonospora echinospora determined in space group I222== |
| - | + | <StructureSection load='3d0q' size='340' side='right'caption='[[3d0q]], [[Resolution|resolution]] 2.79Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[3d0q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D0Q FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene></td></tr> | |
| - | - | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3d0r|3d0r]]</div></td></tr> | |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">calG3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0q OCA], [https://pdbe.org/3d0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d0q RCSB], [https://www.ebi.ac.uk/pdbsum/3d0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0q ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/3d0q_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d0q ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The enediyne antibiotic calicheamicin (CLM) gamma(1)(I) is a prominent antitumor agent that is targeted to DNA by a novel aryltetrasaccharide comprised of an aromatic unit and four unusual carbohydrates. Herein we report the heterologous expression and the biochemical characterization of the two "internal" glycosyltransferases CalG3 and CalG2 and the structural elucidation of an enediyne glycosyltransferase (CalG3). In conjunction with the previous characterization of the "external" CLM GTs CalG1 and CalG4, this study completes the functional assignment of all four CLM GTs, extends the utility of enediyne GT-catalyzed reaction reversibility, and presents conclusive evidence of a sequential glycosylation pathway in CLM biosynthesis. This work also reveals the common GT-B structural fold can now be extended to include enediyne GTs. | ||
| - | + | Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis.,Zhang C, Bitto E, Goff RD, Singh S, Bingman CA, Griffith BR, Albermann C, Phillips GN Jr, Thorson JS Chem Biol. 2008 Aug 25;15(8):842-53. PMID:18721755<ref>PMID:18721755</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3d0q" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Bingman, C A | + | __TOC__ |
| - | [[Category: Bitto, E | + | </StructureSection> |
| - | [[Category: | + | [[Category: Atcc 15837]] |
| - | [[Category: Singh, S | + | [[Category: Large Structures]] |
| - | [[Category: Wesenberg, G E | + | [[Category: Bingman, C A]] |
| + | [[Category: Bitto, E]] | ||
| + | [[Category: Phillips, G N]] | ||
| + | [[Category: Singh, S]] | ||
| + | [[Category: Wesenberg, G E]] | ||
[[Category: Calicheamicin synthesis]] | [[Category: Calicheamicin synthesis]] | ||
[[Category: Enediyne antibiotic]] | [[Category: Enediyne antibiotic]] | ||
[[Category: Glycosyltransferase]] | [[Category: Glycosyltransferase]] | ||
| - | + | [[Category: Transferase]] | |
| - | + | ||
Current revision
Crystal structure of calG3 from Micromonospora echinospora determined in space group I222
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