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3d2h

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{{Seed}}
 
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[[Image:3d2h.png|left|200px]]
 
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==Structure of berberine bridge enzyme from Eschscholzia californica, monoclinic crystal form==
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The line below this paragraph, containing "STRUCTURE_3d2h", creates the "Structure Box" on the page.
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<StructureSection load='3d2h' size='340' side='right'caption='[[3d2h]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3d2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/California_poppy California poppy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D2H FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FSH:(2R,3S,4S)-5-[(4R)-6,7-dimethyl-2,3,5-trioxo-1H-spiro[imidazolidine-4,2-quinoxalin]-4(3H)-yl]-2,3,4-trihydroxypentyl-adenosine+diphosphate'>FSH</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3d2d|3d2d]], [[3d2j|3d2j]]</div></td></tr>
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{{STRUCTURE_3d2h| PDB=3d2h | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BBE1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3467 California poppy])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Reticuline_oxidase Reticuline oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.21.3.3 1.21.3.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d2h OCA], [https://pdbe.org/3d2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d2h RCSB], [https://www.ebi.ac.uk/pdbsum/3d2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d2h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/RETO_ESCCA RETO_ESCCA]] Essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Catalyzes the stereospecific conversion of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon of (S)-scoulerine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/3d2h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d2h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Berberine bridge enzyme catalyzes the conversion of (S)-reticuline to (S)-scoulerine by formation of a carbon-carbon bond between the N-methyl group and the phenolic ring. We elucidated the structure of berberine bridge enzyme from Eschscholzia californica and determined the kinetic rates for three active site protein variants. Here we propose a catalytic mechanism combining base-catalyzed proton abstraction with concerted carbon-carbon coupling accompanied by hydride transfer from the N-methyl group to the N5 atom of the FAD cofactor.
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===Structure of berberine bridge enzyme from Eschscholzia californica, monoclinic crystal form===
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A concerted mechanism for berberine bridge enzyme.,Winkler A, Lyskowski A, Riedl S, Puhl M, Kutchan TM, Macheroux P, Gruber K Nat Chem Biol. 2008 Dec;4(12):739-41. Epub 2008 Oct 26. PMID:18953357<ref>PMID:18953357</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3d2h" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18953357}}, adds the Publication Abstract to the page
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*[[Reticuline oxidase|Reticuline oxidase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18953357 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18953357}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: California poppy]]
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3D2H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Eschscholzia_californica Eschscholzia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D2H OCA].
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[[Category: Large Structures]]
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==Reference==
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A concerted mechanism for berberine bridge enzyme., Winkler A, Lyskowski A, Riedl S, Puhl M, Kutchan TM, Macheroux P, Gruber K, Nat Chem Biol. 2008 Dec;4(12):739-41. Epub 2008 Oct 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18953357 18953357]
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[[Category: Eschscholzia californica]]
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[[Category: Reticuline oxidase]]
[[Category: Reticuline oxidase]]
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[[Category: Single protein]]
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[[Category: Gruber, K]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Lyskowski, A]]
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[[Category: Macheroux, P]]
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[[Category: Winkler, A]]
[[Category: Alakloid biosynthesis]]
[[Category: Alakloid biosynthesis]]
[[Category: Bi-covalent flavinylation]]
[[Category: Bi-covalent flavinylation]]
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: P-cresol methylhydroxylase superfamily]]
[[Category: P-cresol methylhydroxylase superfamily]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 20:49:21 2008''
 

Current revision

Structure of berberine bridge enzyme from Eschscholzia californica, monoclinic crystal form

PDB ID 3d2h

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