1gc3

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[[Image:1gc3.jpg|left|200px]]
[[Image:1gc3.jpg|left|200px]]
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{{Structure
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|PDB= 1gc3 |SIZE=350|CAPTION= <scene name='initialview01'>1gc3</scene>, resolution 3.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1gc3", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span>
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1gc3| PDB=1gc3 | SCENE= }}
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|RELATEDENTRY=[[1bjw|1BJW]], [[1bkg|1BKG]], [[5bj4|5BJ4]], [[1gc4|1GC4]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc3 OCA], [http://www.ebi.ac.uk/pdbsum/1gc3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gc3 RCSB]</span>
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}}
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'''THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 2 COMPLEXED WITH TRYPTOPHAN'''
'''THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 2 COMPLEXED WITH TRYPTOPHAN'''
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[[Category: Nakai, T.]]
[[Category: Nakai, T.]]
[[Category: Ura, H.]]
[[Category: Ura, H.]]
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[[Category: aminotransferase]]
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[[Category: Aminotransferase]]
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[[Category: dual-substrate enzyme]]
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[[Category: Dual-substrate enzyme]]
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[[Category: pyridoxal enzyme]]
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[[Category: Pyridoxal enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:23:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:41:12 2008''
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Revision as of 14:23, 2 May 2008

Image:1gc3.jpg

Template:STRUCTURE 1gc3

THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 2 COMPLEXED WITH TRYPTOPHAN


Overview

Aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 (ttAspAT), has been believed to be specific for an acidic substrate. However, stepwise introduction of mutations in the active-site residues finally changed its substrate specificity to that of a dual-substrate enzyme. The final mutant, [S15D, T17V, K109S, S292R] ttAspAT, is active toward both acidic and hydrophobic substrates. During the course of stepwise mutation, the activities toward acidic and hydrophobic substrates changed independently. The introduction of a mobile Arg292* residue into ttAspAT was the key step in the change to a "dual-substrate" enzyme. The substrate recognition mechanism of this thermostable "dual-substrate" enzyme was confirmed by X-ray crystallography. This work together with previous studies on various enzymes suggest that this unique "dual-substrate recognition" mechanism is a feature of not only aminotransferases but also other enzymes.

About this Structure

1GC3 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Substrate recognition mechanism of thermophilic dual-substrate enzyme., Ura H, Nakai T, Kawaguchi SI, Miyahara I, Hirotsu K, Kuramitsu S, J Biochem. 2001 Jul;130(1):89-98. PMID:11432784 Page seeded by OCA on Fri May 2 17:23:58 2008

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