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3dtp

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{{Seed}}
 
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[[Image:3dtp.png|left|200px]]
 
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==Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP==
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The line below this paragraph, containing "STRUCTURE_3dtp", creates the "Structure Box" on the page.
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<SX load='3dtp' size='340' side='right' viewer='molstar' caption='[[3dtp]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3dtp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Avicularia_avicularia Avicularia avicularia] and [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DTP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1i84|1i84]], [[2fxm|2fxm]], [[1b7t|1b7t]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MYH7, MYHCB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK]), MYL6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
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{{STRUCTURE_3dtp| PDB=3dtp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dtp OCA], [https://pdbe.org/3dtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dtp RCSB], [https://www.ebi.ac.uk/pdbsum/3dtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dtp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/MYH11_CHICK MYH11_CHICK]] Muscle contraction. [[https://www.uniprot.org/uniprot/MYL6_CHICK MYL6_CHICK]] Regulatory light chain of myosin. Does not bind calcium.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/3dtp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dtp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Muscle contraction involves the interaction of the myosin heads of the thick filaments with actin subunits of the thin filaments. Relaxation occurs when this interaction is blocked by molecular switches on these filaments. In many muscles, myosin-linked regulation involves phosphorylation of the myosin regulatory light chains (RLCs). Electron microscopy of vertebrate smooth muscle myosin molecules (regulated by phosphorylation) has provided insight into the relaxed structure, revealing that myosin is switched off by intramolecular interactions between its two heads, the free head and the blocked head. Three-dimensional reconstruction of frozen-hydrated specimens revealed that this asymmetric head interaction is also present in native thick filaments of tarantula striated muscle. Our goal in this study was to elucidate the structural features of the tarantula filament involved in phosphorylation-based regulation. A new reconstruction revealed intra- and intermolecular myosin interactions in addition to those seen previously. To help interpret the interactions, we sequenced the tarantula RLC and fitted an atomic model of the myosin head that included the predicted RLC atomic structure and an S2 (subfragment 2) crystal structure to the reconstruction. The fitting suggests one intramolecular interaction, between the cardiomyopathy loop of the free head and its own S2, and two intermolecular interactions, between the cardiac loop of the free head and the essential light chain of the blocked head and between the Leu305-Gln327 interaction loop of the free head and the N-terminal fragment of the RLC of the blocked head. These interactions, added to those previously described, would help switch off the thick filament. Molecular dynamics simulations suggest how phosphorylation could increase the helical content of the RLC N-terminus, weakening these interactions, thus releasing both heads and activating the thick filament.
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===Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP===
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Three-dimensional reconstruction of tarantula myosin filaments suggests how phosphorylation may regulate myosin activity.,Alamo L, Wriggers W, Pinto A, Bartoli F, Salazar L, Zhao FQ, Craig R, Padron R J Mol Biol. 2008 Dec 26;384(4):780-97. Epub 2008 Oct 14. PMID:18951904<ref>PMID:18951904</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3dtp" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18951904}}, adds the Publication Abstract to the page
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18951904 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18951904}}
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__TOC__
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</SX>
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==About this Structure==
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3DTP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Avicularia_avicularia Avicularia avicularia], [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Gallus_gallus,_homo_sapiens Gallus gallus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DTP OCA].
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==Reference==
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Three-dimensional reconstruction of tarantula myosin filaments suggests how phosphorylation may regulate myosin activity., Alamo L, Wriggers W, Pinto A, Bartoli F, Salazar L, Zhao FQ, Craig R, Padron R, J Mol Biol. 2008 Dec 26;384(4):780-97. Epub 2008 Oct 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18951904 18951904]
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Refined model of the 10S conformation of smooth muscle myosin by cryo-electron microscopy 3D image reconstruction., Liu J, Wendt T, Taylor D, Taylor K, J Mol Biol. 2003 Jun 20;329(5):963-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12798686 12798686]
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Crystal structures of human cardiac beta-myosin II S2-Delta provide insight into the functional role of the S2 subfragment., Blankenfeldt W, Thoma NH, Wray JS, Gautel M, Schlichting I, Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17713-7. Epub 2006 Nov 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17095604 17095604]
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Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head., Houdusse A, Kalabokis VN, Himmel D, Szent-Gyorgyi AG, Cohen C, Cell. 1999 May 14;97(4):459-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10338210 10338210]
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[[Category: Avicularia avicularia]]
[[Category: Avicularia avicularia]]
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[[Category: Gallus gallus]]
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[[Category: Chick]]
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[[Category: Gallus gallus, homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Alamo, L]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Bartoli, F]]
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[[Category: Craig, R]]
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[[Category: Padron, R]]
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[[Category: Pinto, A]]
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[[Category: Salazar, L]]
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[[Category: Wriggers, W]]
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[[Category: Zhao, F Q]]
[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
[[Category: Contractile protein]]
[[Category: Contractile protein]]
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[[Category: Regulatory light chain]]
[[Category: Regulatory light chain]]
[[Category: Smooth muscle]]
[[Category: Smooth muscle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 20:05:19 2008''
 

Current revision

Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP

3dtp, resolution 20.00Å

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