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7fbs
From Proteopedia
(Difference between revisions)
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==structure of a channel== | ==structure of a channel== | ||
| - | <StructureSection load='7fbs' size='340' side='right'caption='[[7fbs]]' scene=''> | + | <StructureSection load='7fbs' size='340' side='right'caption='[[7fbs]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FBS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7fbs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FBS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fbs OCA], [https://pdbe.org/7fbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fbs RCSB], [https://www.ebi.ac.uk/pdbsum/7fbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fbs ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4Y4:1-[2-[(2R)-2-oxidanyl-3-(propylamino)propoxy]phenyl]-3-phenyl-propan-1-one'>4Y4</scene>, <scene name='pdbligand=6OU:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl]+(~{Z})-octadec-9-enoate'>6OU</scene>, <scene name='pdbligand=9Z9:(3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en'>9Z9</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fbs OCA], [https://pdbe.org/7fbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fbs RCSB], [https://www.ebi.ac.uk/pdbsum/7fbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fbs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/SCN5A_RAT SCN5A_RAT]] This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels.[UniProtKB:Q14524][UniProtKB:Q9JJV9] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The heartbeat is initiated by voltage-gated sodium channel NaV1.5, which opens rapidly and triggers the cardiac action potential; however, the structural basis for pore opening remains unknown. Here, we blocked fast inactivation with a mutation and captured the elusive open-state structure. The fast inactivation gate moves away from its receptor, allowing asymmetric opening of pore-lining S6 segments, which bend and rotate at their intracellular ends to dilate the activation gate to approximately 10 A diameter. Molecular dynamics analyses predict physiological rates of Na(+) conductance. The open-state pore blocker propafenone binds in a high-affinity pose, and drug-access pathways are revealed through the open activation gate and fenestrations. Comparison with mutagenesis results provides a structural map of arrhythmia mutations that target the activation and fast inactivation gates. These results give atomic-level insights into molecular events that underlie generation of the action potential, open-state drug block, and fast inactivation of cardiac sodium channels, which initiate the heartbeat. | ||
| + | |||
| + | Open-state structure and pore gating mechanism of the cardiac sodium channel.,Jiang D, Banh R, Gamal El-Din TM, Tonggu L, Lenaeus MJ, Pomes R, Zheng N, Catterall WA Cell. 2021 Sep 4. pii: S0092-8674(21)00995-8. doi: 10.1016/j.cell.2021.08.021. PMID:34520724<ref>PMID:34520724</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7fbs" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Catterall | + | [[Category: Catterall, W A]] |
| - | [[Category: Jiang | + | [[Category: Jiang, D J]] |
| + | [[Category: Four-domain]] | ||
| + | [[Category: Ion channel]] | ||
| + | [[Category: Membrane protein]] | ||
Current revision
structure of a channel
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