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3ecq

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{{Seed}}
 
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[[Image:3ecq.png|left|200px]]
 
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==Endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae: SeMet structure==
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The line below this paragraph, containing "STRUCTURE_3ecq", creates the "Structure Box" on the page.
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<StructureSection load='3ecq' size='340' side='right'caption='[[3ecq]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ecq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Strr6 Strr6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ECQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3ecq| PDB=3ecq | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spr0328 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=171101 STRR6])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endo-alpha-N-acetylgalactosaminidase Endo-alpha-N-acetylgalactosaminidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.97 3.2.1.97] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ecq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ecq OCA], [https://pdbe.org/3ecq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ecq RCSB], [https://www.ebi.ac.uk/pdbsum/3ecq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ecq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/GH101_STRR6 GH101_STRR6]] Is involved in the breakdown of mucin-type O-linked glycans. Specifically removes the T-antigen disaccharide (Gal-beta-1,3-GalNAc-alpha) from extracellular host glycoproteins. Is representative of a broadly important class of virulence factors.<ref>PMID:18784084</ref> <ref>PMID:19788271</ref> <ref>PMID:21877</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/3ecq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ecq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Streptococcus pneumoniae endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase from family GH101 involved in the breakdown of mucin type O-linked glycans. The 189-kDa mature enzyme specifically hydrolyzes the T-antigen disaccharide from extracellular host glycoproteins and is representative of a broadly important class of virulence factors that have remained structurally uncharacterized due to their large size and highly modular nature. Here we report a 2.9 angstroms resolution crystal structure that remarkably captures the multidomain architecture and characterizes a catalytic center unexpectedly resembling that of alpha-amylases. Our analysis presents a complete model of glycoprotein recognition and provides a basis for the structure-based design of novel Streptococcus vaccines and therapeutics.
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===Endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae: SeMet structure===
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The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae: a target for structure-based vaccine design.,Caines ME, Zhu H, Vuckovic M, Willis LM, Withers SG, Wakarchuk WW, Strynadka NC J Biol Chem. 2008 Nov 14;283(46):31279-83. Epub 2008 Sep 10. PMID:18784084<ref>PMID:18784084</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18784084}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ecq" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18784084 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_18784084}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Endo-alpha-N-acetylgalactosaminidase]]
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3ECQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ECQ OCA].
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[[Category: Large Structures]]
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[[Category: Strr6]]
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==Reference==
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[[Category: Caines, M E.C]]
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The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae: a target for structure-based vaccine design., Caines ME, Zhu H, Vuckovic M, Willis LM, Withers SG, Wakarchuk WW, Strynadka NC, J Biol Chem. 2008 Nov 14;283(46):31279-83. Epub 2008 Sep 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18784084 18784084]
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[[Category: Strynadka, N C.J]]
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[[Category: Glycopeptide alpha-N-acetylgalactosaminidase]]
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[[Category: Vuckovic, M]]
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[[Category: Single protein]]
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[[Category: Zhu, H]]
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[[Category: Streptococcus pneumoniae]]
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[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
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[[Category: Cell wall]]
[[Category: Cell wall]]
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[[Category: Hydrolase]]
[[Category: Peptidoglycan-anchor]]
[[Category: Peptidoglycan-anchor]]
[[Category: Secreted]]
[[Category: Secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 31 20:19:32 2008''
 

Current revision

Endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae: SeMet structure

PDB ID 3ecq

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