Barnase

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[[Image:1bni.png|left|200px|thumb|Crystal Structure of Barnase [[1bni]]]]
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<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
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{{STRUCTURE_1bni| PDB=1bni | SIZE=400| SCENE=Barnase/Cv/1 |right|CAPTION=Barnase [[1bni]] }}
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'''Barnase''' (BA, 'BActerial RiboNucleASE') is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
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== Function ==
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{{TOC limit|limit=2}}
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'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
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== 3D Structures of Barnase ==
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== Relevance ==
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[[3kch]], [[2f4y]] – BA cross-linked<br />
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BA structure was used extensively for studying protein folding.
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[[2f56]] - BA cross-linked + urea<br />
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[[2f5w]] - BA cross-linked + thiourea<br />
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[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
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[[1fw7]], [[1bnr]] – BA - NMR<br />
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[[2kf3]] – BA (mutant) – NMR<br />
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[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
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[[1yvs]] – BA domain swapped<br />
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[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
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[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide
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===BA + BST===
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== Structural highlights ==
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[[3da7]] - BA (mutant) + BST<br />
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BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
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[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
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[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
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== 3D Structures of Barnase ==
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[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />
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[[Barnase 3D structures]]
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[[2c4b]] – BA/MCOEETI (mutant)
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</StructureSection>
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Barnase complex with tetranucleotide (PDB code 1brn)

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References

  1. ↑ Buckle AM, Fersht AR. Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. Biochemistry. 1994 Feb 22;33(7):1644-53. PMID:8110767

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky

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