Barnase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:37, 20 February 2022) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
<StructureSection load='1brn' size='450' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
+
<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
== Function ==
== Function ==
-
'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
+
'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
== Relevance ==
== Relevance ==
Line 11: Line 11:
== Structural highlights ==
== Structural highlights ==
-
BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/3'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
+
BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
-
 
+
-
</StructureSection>
+
== 3D Structures of Barnase ==
== 3D Structures of Barnase ==
 +
[[Barnase 3D structures]]
-
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+
</StructureSection>
-
{{#tree:id=OrganizedByTopic|openlevels=0|
+
-
 
+
-
*Barnase
+
-
 
+
-
**[[3kch]], [[2f4y]] – BA cross-linked<br />
+
-
**[[2f56]] - BA cross-linked + urea<br />
+
-
**[[2f5w]] - BA cross-linked + thiourea<br />
+
-
**[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
+
-
**[[1fw7]], [[1bnr]] – BA - NMR<br />
+
-
**[[2kf3]] – BA (mutant) – NMR<br />
+
-
**[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
+
-
**[[1yvs]] – BA domain swapped<br />
+
-
**[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
+
-
**[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide
+
-
 
+
-
*Barnase + BST
+
-
 
+
-
**[[3da7]] - BA (mutant) + BST<br />
+
-
**[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
+
-
**[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
+
-
**[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />
+
-
**[[2c4b]] – BA/MCOEETI (mutant)
 
-
}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Barnase complex with tetranucleotide (PDB code 1brn)

Drag the structure with the mouse to rotate

References

  1. ↑ Buckle AM, Fersht AR. Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. Biochemistry. 1994 Feb 22;33(7):1644-53. PMID:8110767

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky

Retrieved from "http://52.214.119.220/wiki/index.php/Barnase"
Personal tools