Barnase
From Proteopedia
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== Function == | == Function == | ||
| - | '''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA. | + | '''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA. |
== Relevance == | == Relevance == | ||
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</StructureSection> | </StructureSection> | ||
| - | == 3D Structures of Barnase == | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *Barnase | ||
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| - | **[[3kch]], [[2f4y]] – BA cross-linked<br /> | ||
| - | **[[2f56]] - BA cross-linked + urea<br /> | ||
| - | **[[2f5w]] - BA cross-linked + thiourea<br /> | ||
| - | **[[2f5m]] - BA cross-linked + Bromo-ethanol<br /> | ||
| - | **[[1fw7]], [[1bnr]] – BA - NMR<br /> | ||
| - | **[[2kf3]] – BA (mutant) – NMR<br /> | ||
| - | **[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br /> | ||
| - | **[[1yvs]] – BA domain swapped<br /> | ||
| - | **[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br /> | ||
| - | **[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide | ||
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| - | *Barnase + BST | ||
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| - | **[[3da7]] - BA (mutant) + BST<br /> | ||
| - | **[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br /> | ||
| - | **[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br /> | ||
| - | **[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br /> | ||
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| - | **[[2c4b]] – BA/MCOEETI (mutant) | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Buckle AM, Fersht AR. Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. Biochemistry. 1994 Feb 22;33(7):1644-53. PMID:8110767
