This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7vh2
From Proteopedia
(Difference between revisions)
m (Protected "7vh2" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
==Cryo-EM structure of Machupo virus dimeric polymerase L== | ==Cryo-EM structure of Machupo virus dimeric polymerase L== | ||
| - | <StructureSection load='7vh2' size='340' side='right'caption='[[7vh2]]' scene=''> | + | <StructureSection load='7vh2' size='340' side='right'caption='[[7vh2]], [[Resolution|resolution]] 5.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VH2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7vh2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VH2 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vh2 OCA], [https://pdbe.org/7vh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vh2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vh2 ProSAT]</span></td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7vgq|7vgq]], [[7vh1|7vh1]]</div></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vh2 OCA], [https://pdbe.org/7vh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vh2 RCSB], [https://www.ebi.ac.uk/pdbsum/7vh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vh2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/L_MACHU L_MACHU]] RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Arenaviridae family includes several viruses that cause severe human hemorrhagic fevers with high mortality, with no effective countermeasures currently available. The arenavirus multi-domain L protein is involved in viral transcription and replication and represents a promising target for antiviral drugs. The arenavirus matrix protein Z is a small multi-functional protein that inhibits the activities of the L protein. Here we report the structure of Machupo virus L protein in complex with Z determined by cryo-electron microscopy. The Z protein acts as a staple and binds the L protein with 1:1 stoichiometry at the intersection between the PA-C-like region, RNA-dependent RNA polymerase and PB2-N-like region. Binding of the Z protein may lock the multiple domains of L into a fixed arrangement leading to loss of catalytic activity. These results further our understanding of the inhibitory mechanism of arenavirus replication machinery and provide a novel perspective to develop antiviral drugs. | ||
| + | |||
| + | Structure of Machupo virus polymerase in complex with matrix protein Z.,Ma J, Zhang S, Zhang X Nat Commun. 2021 Oct 25;12(1):6163. doi: 10.1038/s41467-021-26432-3. PMID:34697302<ref>PMID:34697302</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7vh2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Ma J]] | + | [[Category: Ma, J]] |
| - | [[Category: Zhang S]] | + | [[Category: Zhang, S]] |
| - | [[Category: Zhang X]] | + | [[Category: Zhang, X]] |
| + | [[Category: Polymerase]] | ||
| + | [[Category: Viral protein]] | ||
Current revision
Cryo-EM structure of Machupo virus dimeric polymerase L
| |||||||||||
