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3fto
From Proteopedia
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| - | [[Image:3fto.png|left|200px]] | ||
| - | + | ==Crystal structure of OppA in a open conformation== | |
| + | <StructureSection load='3fto' size='340' side='right'caption='[[3fto]], [[Resolution|resolution]] 2.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3fto]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptococcus_hollandicus"_scholl_1891 "streptococcus hollandicus" scholl 1891]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FTO FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3drf|3drf]], [[3drg|3drg]], [[3drh|3drh]], [[3dri|3dri]], [[3drj|3drj]], [[3drk|3drk]]</div></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">llmg_0701, oppA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1359 "Streptococcus hollandicus" Scholl 1891])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fto OCA], [https://pdbe.org/3fto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fto RCSB], [https://www.ebi.ac.uk/pdbsum/3fto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fto ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/3fto_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fto ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lactococcus lactis is a promising host for (membrane) protein overproduction. Here, we describe a protocol for incorporation of selenomethionine (SeMet) into proteins expressed in L. lactis. Incorporation efficiencies of SeMet in the membrane protein complex OpuA (an ABC transporter) and the soluble protein OppA, both from L. lactis, were monitored by mass spectrometry. Both proteins incorporated SeMet with high efficiencies (>90%), which greatly extends the usefulness of the expression host L. lactis for X-ray crystallography purposes. The crystal structure of ligand-free OppA was determined at 2.4 A resolution by a semiautomatic approach using selenium single-wavelength anomalous diffraction phasing. | ||
| - | + | Selenomethionine incorporation in proteins expressed in Lactococcus lactis.,Berntsson RP, Alia Oktaviani N, Fusetti F, Thunnissen AM, Poolman B, Slotboom DJ Protein Sci. 2009 May;18(5):1121-7. PMID:19388077<ref>PMID:19388077</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3fto" style="background-color:#fffaf0;"></div> | |
| - | + | ||
==See Also== | ==See Also== | ||
| - | *[[ | + | *[[ABC transporter 3D structures|ABC transporter 3D structures]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Berntsson, R P.A | + | [[Category: Streptococcus hollandicus scholl 1891]] |
| - | [[Category: Fusetti, F | + | [[Category: Large Structures]] |
| - | [[Category: Oktaviani, N A | + | [[Category: Berntsson, R P.A]] |
| - | [[Category: Poolman, B | + | [[Category: Fusetti, F]] |
| - | [[Category: Slotboom, D J | + | [[Category: Oktaviani, N A]] |
| - | [[Category: Thunnissen, A M.W H | + | [[Category: Poolman, B]] |
| + | [[Category: Slotboom, D J]] | ||
| + | [[Category: Thunnissen, A M.W H]] | ||
[[Category: Oligo-peptide binding]] | [[Category: Oligo-peptide binding]] | ||
[[Category: Peptide binding protein]] | [[Category: Peptide binding protein]] | ||
[[Category: Venus fly-trap]] | [[Category: Venus fly-trap]] | ||
[[Category: Voluminous binding cavity]] | [[Category: Voluminous binding cavity]] | ||
Current revision
Crystal structure of OppA in a open conformation
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