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2tmp

From Proteopedia

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{{Seed}}
 
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[[Image:2tmp.png|left|200px]]
 
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==N-TERMINAL DOMAIN OF TISSUE INHIBITOR OF METALLOPROTEINASE-2 (N-TIMP-2), NMR, 49 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_2tmp", creates the "Structure Box" on the page.
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<StructureSection load='2tmp' size='340' side='right'caption='[[2tmp]], [[NMR_Ensembles_of_Models | 49 NMR models]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2tmp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2TMP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2tmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tmp OCA], [https://pdbe.org/2tmp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2tmp RCSB], [https://www.ebi.ac.uk/pdbsum/2tmp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2tmp ProSAT]</span></td></tr>
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</table>
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{{STRUCTURE_2tmp| PDB=2tmp | SCENE= }}
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== Function ==
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[[https://www.uniprot.org/uniprot/TIMP2_HUMAN TIMP2_HUMAN]] Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.<ref>PMID:2793861</ref> <ref>PMID:2554304</ref> <ref>PMID:11710594</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tm/2tmp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2tmp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The high resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 (N-TIMP-2) in solution has been determined using multidimensional heteronuclear NMR spectroscopy, with the structural calculations based on an extensive set of constraints, including 3132 nuclear Overhauser effect-based distance constraints, 56 hydrogen bond constraints, and 220 torsion angle constraints (an average of 26.9 constraints/residue). The core of the protein consists of a five-stranded beta-barrel that is homologous to the beta-barrel found in the oligosaccharide/oligonucleotide binding protein fold. The binding site for the catalytic domain of matrix metalloproteinases-3 (N-MMP-3) on N-TIMP-2 has been mapped by determining the changes in chemical shifts on complex formation for signals from the protein backbone (15N, 13C, and 1H). This approach identified a discrete N-MMP-3 binding site on N-TIMP-2 composed of the N terminus of the protein and the loops between beta-strands AB, CD, and EF. The beta-hairpin formed from strands A and B in N-TIMP-2 is significantly longer than the equivalent structure in TIMP-1, allowing it to make more extensive binding interactions with the MMP catalytic domain. A detailed comparison of the N-TIMP-2 structure with that of TIMP-1 bound to N-MMP-3 (Gomis-Ruth, F.-X., Maskos, K., Betz, M., Bergner, A., Huber, R., Suzuki, K., Yoshida, N., Nagase, H. , Brew, K., Bourne, G. P., Bartunik, H. &amp; Bode, W. (1997) Nature 389, 77-80) revealed that the core beta-barrels are very similar in topology but that the loop connecting beta-strands CD (P67-C72) would need to undergo a large conformational change for TIMP-2 to bind in a similar manner to TIMP-1.
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===N-TERMINAL DOMAIN OF TISSUE INHIBITOR OF METALLOPROTEINASE-2 (N-TIMP-2), NMR, 49 STRUCTURES===
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High resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 and characterization of its interaction site with matrix metalloproteinase-3.,Muskett FW, Frenkiel TA, Feeney J, Freedman RB, Carr MD, Williamson RA J Biol Chem. 1998 Aug 21;273(34):21736-43. PMID:9705310<ref>PMID:9705310</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_9705310}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2tmp" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 9705310 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9705310}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human]]
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2TMP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TMP OCA].
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[[Category: Large Structures]]
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[[Category: Carr, M D]]
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==Reference==
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[[Category: Feeney, J]]
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High resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 and characterization of its interaction site with matrix metalloproteinase-3., Muskett FW, Frenkiel TA, Feeney J, Freedman RB, Carr MD, Williamson RA, J Biol Chem. 1998 Aug 21;273(34):21736-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9705310 9705310]
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[[Category: Freedman, R B]]
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[[Category: Homo sapiens]]
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[[Category: Frenkiel, T A]]
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[[Category: Single protein]]
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[[Category: Muskett, F W]]
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[[Category: Carr, M D.]]
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[[Category: Williamson, R A]]
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[[Category: Feeney, J.]]
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[[Category: Metalloprotease inhibitor]]
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[[Category: Freedman, R B.]]
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[[Category: Frenkiel, T A.]]
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[[Category: Muskett, F W.]]
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[[Category: Williamson, R A.]]
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[[Category: Metalloproteinase inhibitor]]
[[Category: Metalloproteinase inhibitor]]
[[Category: Ob protein fold]]
[[Category: Ob protein fold]]
[[Category: Timp]]
[[Category: Timp]]
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Current revision

N-TERMINAL DOMAIN OF TISSUE INHIBITOR OF METALLOPROTEINASE-2 (N-TIMP-2), NMR, 49 STRUCTURES

PDB ID 2tmp

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