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6p00
From Proteopedia
(Difference between revisions)
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==RebH Variant 8F, Tryptamine 6-halogenase== | ==RebH Variant 8F, Tryptamine 6-halogenase== | ||
| - | <StructureSection load='6p00' size='340' side='right'caption='[[6p00]]' scene=''> | + | <StructureSection load='6p00' size='340' side='right'caption='[[6p00]], [[Resolution|resolution]] 2.25Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P00 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6p00]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"nocardia_aerocolonigenes"_(shinobu_and_kawato_1960)_pridham_1970 "nocardia aerocolonigenes" (shinobu and kawato 1960) pridham 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6P00 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rebH, rbmJ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 "Nocardia aerocolonigenes" (Shinobu and Kawato 1960) Pridham 1970])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tryptophan_7-halogenase Tryptophan 7-halogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.19.9 1.14.19.9] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6p00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p00 OCA], [https://pdbe.org/6p00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6p00 RCSB], [https://www.ebi.ac.uk/pdbsum/6p00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6p00 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/REBH_NOCAE REBH_NOCAE]] Involved in the biosynthesis of the indolocarbazole antitumor agent rebeccamycin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chlorotryptophan. The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:11983340</ref> <ref>PMID:15743914</ref> <ref>PMID:17260957</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Andorfer | + | [[Category: Tryptophan 7-halogenase]] |
| - | [[Category: Lewis | + | [[Category: Andorfer, M C]] |
| - | [[Category: Sukumar N]] | + | [[Category: Lewis, J C]] |
| + | [[Category: Sukumar, N]] | ||
| + | [[Category: Directed evolution]] | ||
| + | [[Category: Flavin]] | ||
| + | [[Category: Flavoprotein]] | ||
| + | [[Category: Halogenase]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 07:09, 16 March 2022
RebH Variant 8F, Tryptamine 6-halogenase
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