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6yrn
From Proteopedia
(Difference between revisions)
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==Structure of the Chlamydomonas reinhardtii SAS-6 coiled-coil domain, P2 crystal form== | ==Structure of the Chlamydomonas reinhardtii SAS-6 coiled-coil domain, P2 crystal form== | ||
| - | <StructureSection load='6yrn' size='340' side='right'caption='[[6yrn]]' scene=''> | + | <StructureSection load='6yrn' size='340' side='right'caption='[[6yrn]], [[Resolution|resolution]] 2.43Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YRN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yrn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YRN FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yrn OCA], [https://pdbe.org/6yrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yrn RCSB], [https://www.ebi.ac.uk/pdbsum/6yrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yrn ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CrSAS-6, CHLRE_12g516950v5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yrn OCA], [https://pdbe.org/6yrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yrn RCSB], [https://www.ebi.ac.uk/pdbsum/6yrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yrn ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9-fold radial symmetry imparted by a likewise symmetrical central scaffold, the cartwheel. The spindle assembly abnormal protein 6 (SAS-6) self-assembles into 9-fold radially symmetric ring-shaped oligomers that stack via an unknown mechanism to form the cartwheel. Here, we uncover a homo-oligomerization interaction mediated by the coiled-coil domain of SAS-6. Crystallographic structures of Chlamydomonas reinhardtii SAS-6 coiled-coil complexes suggest this interaction is asymmetric, thereby imparting polarity to the cartwheel. Using a cryoelectron microscopy (cryo-EM) reconstitution assay, we demonstrate that amino acid substitutions disrupting this asymmetric association also impair SAS-6 ring stacking. Our work raises the possibility that the asymmetric interaction inherent to SAS-6 coiled-coil provides a polar element for cartwheel assembly, which may assist the establishment of the centriolar proximal-distal axis. | ||
| + | |||
| + | Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel.,Kantsadi AL, Hatzopoulos GN, Gonczy P, Vakonakis I Structure. 2022 Feb 22. pii: S0969-2126(22)00041-7. doi:, 10.1016/j.str.2022.02.005. PMID:35240058<ref>PMID:35240058</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yrn" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Chlre]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Kantsadi | + | [[Category: Kantsadi, A L]] |
| - | [[Category: Vakonakis I]] | + | [[Category: Vakonakis, I]] |
| + | [[Category: Alpha helical]] | ||
| + | [[Category: Cartwheel]] | ||
| + | [[Category: Centriole]] | ||
| + | [[Category: Centrosome]] | ||
| + | [[Category: Coiled coil]] | ||
| + | [[Category: Complex]] | ||
| + | [[Category: Structural protein]] | ||
Revision as of 07:12, 16 March 2022
Structure of the Chlamydomonas reinhardtii SAS-6 coiled-coil domain, P2 crystal form
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