This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3gt3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3gt3" [edit=sysop:move=sysop])
Current revision (07:53, 16 March 2022) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3gt3.png|left|200px]]
 
-
{{STRUCTURE_3gt3| PDB=3gt3 | SCENE= }}
+
==Structure of proteinase K with the mad triangle B3C==
 +
<StructureSection load='3gt3' size='340' side='right'caption='[[3gt3]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3gt3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GT3 FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRV:5-AMINO-2,4,6-TRIBROMOBENZENE-1,3-DICARBOXYLIC+ACID'>BRV</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gt4|3gt4]], [[3e3d|3e3d]], [[3e3s|3e3s]], [[3e3t|3e3t]]</div></td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gt3 OCA], [https://pdbe.org/3gt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gt3 RCSB], [https://www.ebi.ac.uk/pdbsum/3gt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gt3 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[[https://www.uniprot.org/uniprot/PRTK_TRIAL PRTK_TRIAL]] Hydrolyzes keratin at aromatic and hydrophobic residues.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/3gt3_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gt3 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Experimental phasing is an essential technique for the solution of macromolecular structures. Since many heavy-atom ion soaks suffer from nonspecific binding, a novel class of compounds has been developed that combines heavy atoms with functional groups for binding to proteins. The phasing tool 5-amino-2,4,6-tribromoisophthalic acid (B3C) contains three functional groups (two carboxylate groups and one amino group) that interact with proteins via hydrogen bonds. Three Br atoms suitable for anomalous dispersion phasing are arranged in an equilateral triangle and are thus readily identified in the heavy-atom substructure. B3C was incorporated into proteinase K and a multiwavelength anomalous dispersion (MAD) experiment at the Br K edge was successfully carried out. Radiation damage to the bromine-carbon bond was investigated. A comparison with the phasing tool I3C that contains three I atoms for single-wavelength anomalous dispersion (SAD) phasing was also carried out.
-
===Structure of proteinase K with the mad triangle B3C===
+
The magic triangle goes MAD: experimental phasing with a bromine derivative.,Beck T, Gruene T, Sheldrick GM Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):374-80. Epub 2010, Mar 24. PMID:20382990<ref>PMID:20382990</ref>
-
{{ABSTRACT_PUBMED_19020357}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3gt3" style="background-color:#fffaf0;"></div>
-
==About this Structure==
+
==See Also==
-
[[3gt3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Engyodontium_album Engyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GT3 OCA].
+
*[[Proteinase 3D structures|Proteinase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:019020357</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Engyodontium album]]
[[Category: Engyodontium album]]
 +
[[Category: Large Structures]]
[[Category: Peptidase K]]
[[Category: Peptidase K]]
-
[[Category: Beck, T.]]
+
[[Category: Beck, T]]
-
[[Category: Gruene, T.]]
+
[[Category: Gruene, T]]
-
[[Category: Sheldrick, G M.]]
+
[[Category: Sheldrick, G M]]
[[Category: 5-amino-2]]
[[Category: 5-amino-2]]
[[Category: 6-tribromoisophthalic acid]]
[[Category: 6-tribromoisophthalic acid]]

Current revision

Structure of proteinase K with the mad triangle B3C

PDB ID 3gt3

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gt3"
Personal tools