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2vof
From Proteopedia
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| - | [[Image:2vof.jpg|left|200px]] | ||
| - | + | ==Structure of mouse A1 bound to the Puma BH3-domain== | |
| - | + | <StructureSection load='2vof' size='340' side='right'caption='[[2vof]], [[Resolution|resolution]] 1.80Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2vof]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VOF FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |
| - | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |
| - | | | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vog|2vog]], [[2voh|2voh]], [[2voi|2voi]]</div></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vof OCA], [https://pdbe.org/2vof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vof RCSB], [https://www.ebi.ac.uk/pdbsum/2vof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vof ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| + | [[https://www.uniprot.org/uniprot/BBC3_MOUSE BBC3_MOUSE]] Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (By similarity). Regulates ER stress-induced neuronal apoptosis.<ref>PMID:21159964</ref> <ref>PMID:22761832</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vo/2vof_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vof ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Apoptotic pathways are regulated by protein-protein interactions. Interaction of the BH3 domains of proapoptotic Bcl-2 family proteins with the hydrophobic groove of prosurvival proteins is critical. Whereas some BH3 domains bind in a promiscuous manner, others exhibit considerable selectivity and the sequence characteristics that distinguish these activities are unclear. In this study, crystal structures of complexes between the prosurvival protein A1 and the BH3 domains from Puma, Bmf, Bak, and Bid have been solved. The structure of A1 is similar to that of other prosurvival proteins, although features, such as an acidic patch in the binding groove, may allow specific therapeutic modulation of apoptosis. Significant conformational plasticity was observed in the intermolecular interactions and these differences explain some of the variation in affinity. This study, in combination with published data, suggests that interactions between conserved residues demarcate optimal binding. | ||
| - | + | Structural plasticity underpins promiscuous binding of the prosurvival protein A1.,Smits C, Czabotar PE, Hinds MG, Day CL Structure. 2008 May;16(5):818-29. PMID:18462686<ref>PMID:18462686</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2vof" style="background-color:#fffaf0;"></div> | ||
| - | == | + | ==See Also== |
| - | + | *[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]] | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Czabotar, P E | + | __TOC__ |
| - | [[Category: Day, C L | + | </StructureSection> |
| - | [[Category: Hinds, M G | + | [[Category: Large Structures]] |
| - | [[Category: Smits, C | + | [[Category: Lk3 transgenic mice]] |
| - | [[Category: | + | [[Category: Czabotar, P E]] |
| - | [[Category: | + | [[Category: Day, C L]] |
| - | [[Category: | + | [[Category: Hinds, M G]] |
| - | [[Category: | + | [[Category: Smits, C]] |
| - | [[Category: | + | [[Category: Apoptosis]] |
| - | + | [[Category: Bcl-2]] | |
| - | [[Category: | + | [[Category: Bh3]] |
| - | + | [[Category: Mitochondrion]] | |
| - | + | [[Category: Pro-survival]] | |
| + | [[Category: Protein-protein complex]] | ||
Current revision
Structure of mouse A1 bound to the Puma BH3-domain
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