1gve
From Proteopedia
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'''AFLATOXIN ALDEHYDE REDUCTASE (AKR7A1) FROM RAT LIVER''' | '''AFLATOXIN ALDEHYDE REDUCTASE (AKR7A1) FROM RAT LIVER''' | ||
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[[Category: Kozma, E.]] | [[Category: Kozma, E.]] | ||
[[Category: Lapthorn, A J.]] | [[Category: Lapthorn, A J.]] | ||
| - | [[Category: | + | [[Category: Aflatoxin b1]] |
| - | [[Category: | + | [[Category: Akr7 family]] |
| - | [[Category: | + | [[Category: Aldo-keto reductase]] |
| - | [[Category: | + | [[Category: Succinic semialdehyde oxidoreductase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:03:25 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:03, 2 May 2008
AFLATOXIN ALDEHYDE REDUCTASE (AKR7A1) FROM RAT LIVER
Overview
The structure of the rat liver aflatoxin dialdehyde reductase (AKR7A1) has been solved to 1.38-A resolution. Although it shares a similar alpha/beta-barrel structure with other members of the aldo-keto reductase superfamily, AKR7A1 is the first dimeric member to be crystallized. The crystal structure also reveals details of the ternary complex as one subunit of the dimer contains NADP(+) and the inhibitor citrate. Although the underlying catalytic mechanism appears similar to other aldo-keto reductases, the substrate-binding pocket contains several charged amino acids (Arg-231 and Arg-327) that distinguish it from previously characterized aldo-keto reductases with respect to size and charge. These differences account for the substrate specificity for 4-carbon acid-aldehydes such as succinic semialdehyde and 2-carboxybenzaldehyde as well as for the idiosyncratic substrate aflatoxin B(1) dialdehyde of this subfamily of enzymes. Structural differences between the AKR7A1 ternary complex and apoenzyme reveal a significant hinged movement of the enzyme involving not only the loops of the structure but also parts of the alpha/beta-barrel most intimately involved in cofactor binding.
About this Structure
1GVE is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
The crystal structure of rat liver AKR7A1. A dimeric member of the aldo-keto reductase superfamily., Kozma E, Brown E, Ellis EM, Lapthorn AJ, J Biol Chem. 2002 May 3;277(18):16285-93. Epub 2002 Feb 11. PMID:11839745 Page seeded by OCA on Fri May 2 18:03:25 2008
