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2wwx
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==Crystal structure of the SidM/DrrA(GEF/GDF domain)-Rab1(GTPase domain) complex== | ==Crystal structure of the SidM/DrrA(GEF/GDF domain)-Rab1(GTPase domain) complex== | ||
| - | <StructureSection load='2wwx' size='340' side='right' caption='[[2wwx]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='2wwx' size='340' side='right'caption='[[2wwx]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2wwx]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2wwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WWX FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2fol|2fol]]</td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fol|2fol]]</div></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wwx OCA], [https://pdbe.org/2wwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wwx RCSB], [https://www.ebi.ac.uk/pdbsum/2wwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wwx ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/RAB1A_HUMAN RAB1A_HUMAN]] Probably required for transit of protein from the ER through Golgi compartment. Binds GTP and GDP and possesses intrinsic GTPase activity. [[https://www.uniprot.org/uniprot/DRRA_LEGPN DRRA_LEGPN]] Virulence effector that plays a key role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a GDP-GTP exchange factor (GEF) for the small GTPase Rab1 (RAB1A, RAB1B or RAB1C), thereby converting Rab1 to an active GTP-bound state, leading to the incorporation of Rab1 into LCVs. Also shows RabGDI displacement factor (GDF) activity; however, this probably represents a passive activity following the GEF activity. Also acts as an adenylyltransferase by mediating the addition of adenosine 5'-monophosphate (AMP) to 'Tyr-77' of host RAB1B, thereby rendering RAB1B constitutively active. Also has adenylyltransferase activity towards Rab6 and Rab35. Also displays guanylyltransferase activity by mediating the addition of guanosine 5'-monophosphate (GMP) to host RAB1B in vitro; however such activity remains uncertain in vivo. Specifically binds phosphatidylinositol 4-phosphate (PtdIns(4)P) lipids on the cytosolic surface of the phagosomal membrane shortly after infection.<ref>PMID:16906144</ref> <ref>PMID:17952054</ref> <ref>PMID:20651120</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</div> | </div> | ||
<div class="pdbe-citations 2wwx" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2wwx" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Atcc 33152]] | [[Category: Atcc 33152]] | ||
[[Category: Human]] | [[Category: Human]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Lee, D W]] | [[Category: Lee, D W]] | ||
[[Category: Oh, B H]] | [[Category: Oh, B H]] | ||
Current revision
Crystal structure of the SidM/DrrA(GEF/GDF domain)-Rab1(GTPase domain) complex
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