This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3i53
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH)== | ==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH)== | ||
| - | <StructureSection load='3i53' size='340' side='right' caption='[[3i53]], [[Resolution|resolution]] 2.08Å' scene=''> | + | <StructureSection load='3i53' size='340' side='right'caption='[[3i53]], [[Resolution|resolution]] 2.08Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3i53]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3i53]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_carzinostaticus_subsp._neocarzinostaticus"_ishida_et_al. "streptomyces carzinostaticus subsp. neocarzinostaticus" ishida et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I53 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i58|3i58]], [[3i5u|3i5u]], [[3i64|3i64]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i58|3i58]], [[3i5u|3i5u]], [[3i64|3i64]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ncsb1 ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ncsb1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i53 OCA], [https://pdbe.org/3i53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i53 RCSB], [https://www.ebi.ac.uk/pdbsum/3i53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i53 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/NCSB1_STRCZ NCSB1_STRCZ]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 35: | Line 35: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]] | [[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Bruner, S D]] | [[Category: Bruner, S D]] | ||
[[Category: Cooke, H A]] | [[Category: Cooke, H A]] | ||
Revision as of 10:57, 13 April 2022
Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH)
| |||||||||||
