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3iaa

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[[Image:3iaa.png|left|200px]]
 
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==Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP bound form==
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The line below this paragraph, containing "STRUCTURE_3iaa", creates the "Structure Box" on the page.
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<StructureSection load='3iaa' size='340' side='right'caption='[[3iaa]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3iaa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IAA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3iaa| PDB=3iaa | SCENE= }}
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ia7|3ia7]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">calG2, Q8KNE0 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iaa OCA], [https://pdbe.org/3iaa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iaa RCSB], [https://www.ebi.ac.uk/pdbsum/3iaa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iaa ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/3iaa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iaa ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.
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===Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP bound form===
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Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3iaa" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21987796}}, adds the Publication Abstract to the page
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21987796 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21987796}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Atcc 15837]]
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[[3iaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IAA OCA].
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[[Category: Large Structures]]
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[[Category: Bingman, C A]]
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==Reference==
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[[Category: Chang, A]]
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<ref group="xtra">PMID:021987796</ref><references group="xtra"/>
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[[Category: Phillips, G N]]
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[[Category: Micromonospora echinospora]]
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[[Category: Singh, S]]
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[[Category: Bingman, C A.]]
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[[Category: Thorson, J S]]
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[[Category: Chang, A.]]
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[[Category: Phillips, G N.]]
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[[Category: Singh, S.]]
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[[Category: Thorson, J S.]]
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[[Category: Calg2]]
[[Category: Calg2]]
[[Category: Calicheamicin]]
[[Category: Calicheamicin]]

Current revision

Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP bound form

PDB ID 3iaa

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