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7qfz
From Proteopedia
(Difference between revisions)
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==BrxR, a WYL-domain containing transcriptional regulator== | ==BrxR, a WYL-domain containing transcriptional regulator== | ||
| - | <StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]]' scene=''> | + | <StructureSection load='7qfz' size='340' side='right'caption='[[7qfz]], [[Resolution|resolution]] 2.15Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7qfz]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QFZ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qfz OCA], [https://pdbe.org/7qfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qfz RCSB], [https://www.ebi.ac.uk/pdbsum/7qfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qfz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteria are under constant assault by bacteriophages and other mobile genetic elements. As a result, bacteria have evolved a multitude of systems that protect from attack. Genes encoding bacterial defence mechanisms can be clustered into 'defence islands', providing a potentially synergistic level of protection against a wider range of assailants. However, there is a comparative paucity of information on how expression of these defence systems is controlled. Here, we functionally characterize a transcriptional regulator, BrxR, encoded within a recently described phage defence island from a multidrug resistant plasmid of the emerging pathogen Escherichia fergusonii. Using a combination of reporters and electrophoretic mobility shift assays, we discovered that BrxR acts as a repressor. We present the structure of BrxR to 2.15 A, the first structure of this family of transcription factors, and pinpoint a likely binding site for ligands within the WYL-domain. Bioinformatic analyses demonstrated that BrxR-family homologues are widespread amongst bacteria. About half (48%) of identified BrxR homologues were co-localized with a diverse array of known phage defence systems, either alone or clustered into defence islands. BrxR is a novel regulator that reveals a common mechanism for controlling the expression of the bacterial phage defence arsenal. | ||
| + | |||
| + | A widespread family of WYL-domain transcriptional regulators co-localizes with diverse phage defence systems and islands.,Picton DM, Harling-Lee JD, Duffner SJ, Went SC, Morgan RD, Hinton JCD, Blower TR Nucleic Acids Res. 2022 May 20;50(9):5191-5207. doi: 10.1093/nar/gkac334. PMID:35544231<ref>PMID:35544231</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qfz" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Blower | + | [[Category: Blower, T R]] |
| - | [[Category: Picton | + | [[Category: Picton, D M]] |
| + | [[Category: Dna binding]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Phage defence]] | ||
| + | [[Category: Transcription factor]] | ||
Revision as of 08:14, 25 May 2022
BrxR, a WYL-domain containing transcriptional regulator
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