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1h7q
From Proteopedia
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'''DTDP-MANGANESE COMPLEX OF SPSA FROM BACILLUS SUBTILIS''' | '''DTDP-MANGANESE COMPLEX OF SPSA FROM BACILLUS SUBTILIS''' | ||
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[[Category: Davies, G J.]] | [[Category: Davies, G J.]] | ||
[[Category: Tarbouriech, N.]] | [[Category: Tarbouriech, N.]] | ||
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| - | [[Category: | + | [[Category: Transferase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:32:30 2008'' | |
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Revision as of 15:32, 2 May 2008
DTDP-MANGANESE COMPLEX OF SPSA FROM BACILLUS SUBTILIS
Overview
The vast majority of glycosidic-bond synthesis in nature is performed by glycosyltransferases, which use activated glycosides as the sugar donor. Typically, the activated leaving group is a nucleoside phosphate, lipid phosphate or phosphate. The nucleotide-sugar-dependent glycosyltransferases fall into over 50 sequence-based families, with the largest and most widespread family of inverting transferases named family GT-2. Here, we present the three-dimensional crystal structure of SpsA, the first and currently the only structural representative from family GT-2, in complex with both Mn-dTDP and Mg-dTDP at a resolution of 2 A. These structures reveal how SpsA and related enzymes may display nucleotide plasticity and permit a comparison of the catalytic centre of this enzyme with those from related sequence families whose three-dimensional structures have recently been determined. Family GT-2 enzymes, together with enzymes from families 7, 13 and 43, appear to form a clan of related structures with identical catalytic apparatus and reaction mechanism.
About this Structure
1H7Q is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Three-dimensional structures of the Mn and Mg dTDP complexes of the family GT-2 glycosyltransferase SpsA: a comparison with related NDP-sugar glycosyltransferases., Tarbouriech N, Charnock SJ, Davies GJ, J Mol Biol. 2001 Dec 7;314(4):655-61. PMID:11733986 Page seeded by OCA on Fri May 2 18:32:30 2008
