Journal:IUCrJ:S2052252518018274
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| - | <StructureSection load='' size='450' side='right' scene='80/804516/Cv/1' caption=' | + | <StructureSection load='' size='450' side='right' scene='80/804516/Cv/1' caption=''> |
===A cytosine modification mechanism revealed by the ternary complex structure of deoxycytidylate hydroxymethylase from bacteriophage T4 with its cofactor and substrate=== | ===A cytosine modification mechanism revealed by the ternary complex structure of deoxycytidylate hydroxymethylase from bacteriophage T4 with its cofactor and substrate=== | ||
<big>Si Hoon Park, Se Won Suh and Hyun Hyu Song</big> <ref>doi 10.1107/S2052252518018274</ref> | <big>Si Hoon Park, Se Won Suh and Hyun Hyu Song</big> <ref>doi 10.1107/S2052252518018274</ref> | ||
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Crystal structure of the ternary complex of dCMP hydroxylmethylase from bacteriophage T4 (T4dCH) bound with dCMP and tetrahydrofolate was determined at 1.9 Å resolution. The key residues within T4dCH for accommodating a cofactor without the C-terminal tail, an optimized network of ordered water molecules, and hydrophobic gating mechanism for cofactor regulation were clearly identified. | Crystal structure of the ternary complex of dCMP hydroxylmethylase from bacteriophage T4 (T4dCH) bound with dCMP and tetrahydrofolate was determined at 1.9 Å resolution. The key residues within T4dCH for accommodating a cofactor without the C-terminal tail, an optimized network of ordered water molecules, and hydrophobic gating mechanism for cofactor regulation were clearly identified. | ||
| - | <scene name='80/804516/Cv/ | + | <scene name='80/804516/Cv/18'>Close up view substrate, dCMP and cofactor, tetrahydrofolate</scene>. THF (orange) and dCMP (green) are drawn as ball and stick models. Oxygen and nitrogen atoms are colored red and blue, respectively. The ligand-recognizing residues are drawn as ball and stick models. Ionic and hydrogen interactions are drawn by dashed lines. Water molecules are shown as red balls. |
| + | *<scene name='80/804516/Cv/19'>dCMP binding site</scene>. | ||
| + | *<scene name='80/804516/Cv/20'>THF binding site</scene>. | ||
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| + | <scene name='80/804516/Cv/23'>Ternary complex structure of T4dCH with dCMP and THF</scene>. A ribbon diagram showing the ternary complex structure of dimeric T4dCH. The bound dCMP (green) and THF (orange) are drawn using the ball and stick model. | ||
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| + | '''PDB references:''' apo T4dCH (I-SAD phasing), [[6a9b]]; ternary complex (T4dCH–dCMP–THF), [[6a9a]]. | ||
<b>References</b><br> | <b>References</b><br> | ||
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